Recent Submissions

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    Programming Skeletal Muscle Metabolic Flexibility in Offspring of Male Rats in Response to Maternal Consumption of Slow Digesting Carbohydrates during Pregnancy  [Article]

    Pérez Alegre, Mónica; Salto, Rafael; Girón, María D.; Manzano, Manuel; Martín, María J.; Vílchez, Jose D.; Bueno Vargas, Pilar; Andújar Pulido, Eloisa (MDPI, 2020)
    Skeletal muscle plays a relevant role in metabolic flexibility and fuel usage and the associated muscle metabolic inflexibility due to high-fat diets contributing to obesity and type 2 diabetes. Previous research from our ...
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    Crosstalk between chromatin structure, cohesin activity and transcription  [Article]

    Maya Miles, Douglas; Andújar, Eloísa; Pérez Alegre, Mónica; Murillo Pineda, Marina; Barrientos Moreno, Marta; Cabello Lobato, María José; Gómez Marín, Elena; Morillo Huesca, Macarena; Prado, Félix (BMC (part of Springer Nature), 2019)
    Background: A complex interplay between chromatin and topological machineries is critical for genome architec‑ ture and function. However, little is known about these reciprocal interactions, even for cohesin, despite its ...
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    Sumoylation of Smc5 Promotes Error-free Bypass at Damaged Replication Forks  [Article]

    Zapatka, Mariel; Pociño Merino, Irene; Heluani Gahete, Hayat; Bermúdez López, Marcelino; Tarrés, Marc; Ibars, Eva; Solé Soler, Roger; Gutiérrez Escribano, Pilar; Apostolova, Sonia; Casas, Celia; Aragon, Luis; Wellinger, Ralf Erik; Colomina, Neus; Torres Rosell, Jordi (Elsevier (Cell Press): OAJ, 2019)
    Replication of a damaged DNA template can threaten the integrity of the genome, requiring the use of various mechanisms to tolerate DNA lesions. The Smc5/6 complex, together with the Nse2/Mms21 SUMO ligase, plays essential ...
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    What causes an RNA-DNA hybrid to compromise genome integrity?  [Article]

    García Rondón, Ana; Aguilera López, Andrés (Elsevier, 2019)
    Transcription is a source of genome instability that stimulates mutation and recombination. Part of the damage produced by transcription is mediated by R-loops, non-B DNA structures that normally form by the re-annealing ...
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    R Loops: From Physiologicalto Pathological Roles  [Article]

    García Muse, Tatiana; Aguilera López, Andrés (Elsevier, 2019-10-17)
    DNA-RNA hybrids play a physiological role in cellular processes, but often, they represent non-scheduled co-transcriptional structures with a negative impact on transcription, replication and DNA repair. Accumulating ...
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    The DNA damage response acts as a safeguardagainst harmful DNA–RNA hybrids ofdifferent origins  [Article]

    Barroso Ceballos, Sonia Inés; Herrera Moyano, Emilia; Muñoz Sánchez, Sergio; García Rubio, María Luisa; Gómez González, Belén; Aguilera López, Andrés (EMBO Press, 2019-07-24)
    Despite playing physiological roles in specific situations, DNA–RNA hybrids threat genome integrity. To investigate how cells do counteract spontaneous DNA–RNA hybrids, here we screen an siRNA library covering 240 human ...
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    Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability  [Article]

    Zatreanu, Diana; Han, Zhong; Mitter, Richard; Tumini, Emanuela; Williams, Hannah C.; Gregersen, Lea Haarup; Dirac-Svejstrup, A. Barbara; Roma, Stefania; Stewart, Aengus; Aguilera López, Andrés; Svejstrup, Jesper (Elsevier, 2019)
    Although correlations between RNA polymerase II (RNAPII) transcription stress, R-loops, and genome instability have been established, the mechanisms underlying these connections remain poorly understood. Here, we used a ...
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    Homologous Recombination: To Fork and Beyond  [Article]

    Prado Velasco, José Félix (MDPI, 2018)
    Accurate completion of genome duplication is threatened by multiple factors that hamper the advance and stability of the replication forks. Cells need to tolerate many of these blocking lesions to timely complete DNA ...
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    Transcription-mediated replication hindrance: a major driver of genome instability  [Article]

    Gómez González, Belén; Aguilera López, Andrés (Cold Spring Harbor Laboratory Press, 2019)
    Genome replication involves dealing with obstacles that can result from DNA damage but also from chromatin alterations, topological stress, tightly bound proteins or non-B DNA structures such as R loops. Experimental ...
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    Rpd3L and Hda1 histone deacetylases facilitate repair of broken forks by promoting sister chromatid cohesion  [Article]

    Aguilera López, Andrés; Gómez González, Belén; Ortega, Pedro (Nature Research, 2019)
    Genome stability involves accurate replication and DNA repair. Broken replication forks, such as those encountering a nick, lead to double strand breaks (DSBs), which are preferentially repaired by sister-chromatid ...
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    Human Mesenchymal Stem Cells Prevent Neurological Complications of Radiotherapy  [Article]

    Soria Escoms, Bernat; Martín Montalvo, Alejandro; Aguilera García, Yolanda; Mellado, Nuria; López Beas, Javier; Herrera-Herrera, Isabel; López, Escarlata; Barcia, Juan A.; Álvarez Dolado, Manuel; Hmadcha, Abdelkrim; Capilla-González, Vivian (Frontiers Media S.A., 2019-05-16)
    Radiotherapy is a highly effective tool for the treatment of brain cancer. However, radiation also causes detrimental effects in the healthy tissue, leading to neurocognitive sequelae that compromise the quality of life ...
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    Oestrogen receptor β mediates the actions of bisphenol-A on ion channel expression in mouse pancreatic beta cells  [Article]

    Martínez-Pinna, Juan; Marroquí, Laura; Hmadcha, Abdelkrim; López Beas, Javier; Soriano, Sergi; Villar-Pazos, Sabrina; Alonso-Magdalena, Paloma; Santos, Reinaldo S. Dos; Quesada, Iván; Martin, Franz; Soria Escoms, Bernat (Springer Nature, 2019-09)
    Aims/hypothesis: Bisphenol-A (BPA) is a widespread endocrine-disrupting chemical that has been associated with type 2 diabetes development. Low doses of BPA modify pancreatic beta cell function and induce insulin resistance; ...
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    The Cornelia de Lange Syndrome-associated factor NIPBL interacts with BRD4 ET domain for transcription control of a common set of genes  [Article]

    Luna-Peláez, Noelia; March Díaz, Rosana Rocío; Ceballos Chávez, María; Guerrero Martínez, José A.; Grazioli, Paolo; García Gutiérrez, Pablo; Vaccari, Thomas; Massa, Valentina; Reyes , José Carlos; García Domínguez, Mario (Springer Nature, 2019-07-18)
    Mutations in NIPBL are the major cause of Cornelia de Lange Syndrome (CdLS). NIPBL is the cohesin-loading factor and has recently been associated with the BET (bromodomains and extra-terminal (ET) domain) proteins BRD2 and ...
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    Depletion of the MFAP1/SPP381 Splicing Factor Causes R-Loop-Independent Genome Instability  [Article]

    Salas Armenteros, Irene; Barroso Ceballos, Sonia Inés; García Rondón, Ana; Pérez Calero, Mónica; Andújar, Eloísa; Aguilera López, Andrés (Elsevier, 2019)
    THO/TREX is a conserved complex with a role in messenger ribonucleoprotein biogenesis that links gene expression and genome instability. Here, we show that human THO interacts with MFAP1 (microfibrillar-associated protein ...
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    Non-canonical replication initiation: You’re fired!  [Article]

    Ravoitytė, Bazilė; Wellinger, Ralf Erik (Multidisciplinary Digital Publishing Institute (MDPI), 2017)
    The division of prokaryotic and eukaryotic cells produces two cells that inherit a perfect copy of the genetic material originally derived from the mother cell. The initiation of canonical DNA replication must be coordinated ...
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    Histone Mutants Separate R Loop Formation from Genome Instability Induction  [Article]

    García Pichardo, Desiré; Cañas, Juan C.; García Rubio, María Luisa; Gómez González, Belén; García Rondón, Ana; Aguilera López, Andrés (Elsevier, 2017)
    R loops have positive physiological roles, but they can also be deleterious by causing genome instability, and the mechanisms for this are unknown. Here we identified yeast histone H3 and H4 mutations that facilitate R ...
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    Late rDNA Condensation Ensures Timely Cdc14 Release and Coordination of Mitotic Exit Signaling with Nucleolar Segregation  [Article]

    Santos Velázquez, Ana Isabel de los; García de Oya, Inés; Manzano López, Javier; Monje Casas, Fernando (Elsevier, 2017)
    The nucleolus plays a pivotal role in multiple key cellular processes. An illustrative example is the regulation of mitotic exit in Saccharomyces cerevisiae through the nucleolar sequestration of the Cdc14 phosphatase. The ...
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    Human THO–Sin3A interaction reveals new mechanisms to prevent R-loops that cause genome instability  [Article]

    Salas Armenteros, Irene; Pérez Calero, Carmen; Bayona Feliu, Aleix; Tumini, Emanuela; Luna Varo, Rosa María; Aguilera López, Andrés (Wiley-Blackwell, 2017)
    R-loops, formed by co-transcriptional DNA–RNA hybrids and a displaced DNA single strand (ssDNA), fulfill certain positive regulatory roles but are also a source of genomic instability. One key cellular mechanism to prevent ...
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    The Nup84 complex coordinates the DNA damage response to warrant genome integrity  [Article]

    Gaillard, Hélène; Santos Pereira, José María; Aguilera López, Andrés (Oxford University Press, 2019)
    DNA lesions interfere with cellular processes such as transcription and replication and need to be adequately resolved to warrant genome integrity. Beyond their primary role in molecule transport, nuclear pore complexes ...
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    Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways  [Article]

    Klein, Hannah L.; Bačinskaja, Giedré; Che, Jun; Cheblal, Anais; Elango, Rajula; Epshtein, Anastasiya; Fitzgerald, Devon M.; Gómez González, Belén; Khan, Sharik R.; Kumar, Sandeep Mothish; Leland, Bryan A.; Marie, Léa; Aguilera López, Andrés (Shared Science Publishers OG, 2019)
    Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular ...

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