Artículo
Enzyme-Responsive Zr-Based Metal-Organic Frameworks for Controlled Drug Delivery: Taking Advantage of Clickable PEG-Phosphate Ligands
Autor/es | Carrillo Carrión, Carolina
Comaills, Valentine ![]() ![]() ![]() ![]() ![]() ![]() Visiga, Ana M. Gauthier, Benoit R. Khiar, Noureddine |
Departamento | Universidad de Sevilla. Departamento de Biología Celular |
Fecha de publicación | 2023 |
Fecha de depósito | 2024-06-13 |
Publicado en |
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Resumen | We report for the first time the controlled drug release from a nanoscale Zr-based metal-organic framework (MOF), UiO-66, in the presence of the enzyme alkaline phosphatase (ALP). This unprecedented reactivity was possible ... We report for the first time the controlled drug release from a nanoscale Zr-based metal-organic framework (MOF), UiO-66, in the presence of the enzyme alkaline phosphatase (ALP). This unprecedented reactivity was possible thanks to the prior functionalization of the MOF with N3-PEG-PO3 ligands, which were designed for three specific aims: (1) to impart colloidal stability in phosphate-containing media; (2) to endow the MOF with multifunctionality thanks to azide groups for the covalent attachment of an imaging agent by click-chemistry; and (3) to confer stimuli-responsive properties, specifically the selective release of doxorubicin triggered by the enzymatic activity of ALP. Cell studies revealed that the functionalization of the MOF with N3-(PEG)20-PO3 ligands improved their intracellular stability and led to a sustained drug release compared to the bare MOF. More importantly, an enhanced drug release was observed in cells with higher expression of ALP genes (HeLa versus MDA-MB-231 and MCF7), confirming the ALP-responsiveness of the system inside living cells. |
Agencias financiadoras | Ministerio de Ciencia, Innovación y Universidades (MICINN). España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Junta de Andalucía Asociación Española Contra el Cáncer Marie Sklodowska-Curie Individual Fellowship European Cooperation in Science and Technology (COST) |
Identificador del proyecto | RYC-2019-027527-I
![]() PID2020-119949RB-I00 ![]() PY20_00882 ![]() CV20-04221 ![]() FQM-313 ![]() INVES20033COMA ![]() 101026137 ![]() CA-18132 ![]() |
Cita | Carrillo Carrión, C., Comaills, V., Visiga, A.M., Gauthier, B.R. y Khiar, N. (2023). Enzyme-Responsive Zr-Based Metal-Organic Frameworks for Controlled Drug Delivery: Taking Advantage of Clickable PEG-Phosphate Ligands. ACS Applied Materials and Interfaces, 15 (23), 27600-27611. https://doi.org/10.1021/acsami.3c03230. |
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