Now showing items 1-10 of 11
The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage
(Public Library of Science, 2017)
RNA-DNA hybrids are naturally occurring obstacles that must be overcome by the DNA replication machinery. In the absence of RNase H enzymes, RNA-DNA hybrids accumulate, resulting in replication stress, DNA damage and ...
The replication checkpoint protects fork stability by releasing transcribed genes from nuclear pores
Transcription hinders replication fork progression and stability, and the Mec1/ATR checkpoint protects fork integrity. Examining checkpoint-dependent mechanisms controlling fork stability, we find that fork reversal and ...
Transcription-mediated replication hindrance: a major driver of genome instability
(Cold Spring Harbor Laboratory Press, 2019)
Genome replication involves dealing with obstacles that can result from DNA damage but also from chromatin alterations, topological stress, tightly bound proteins or non-B DNA structures such as R loops. Experimental ...
Rpd3L and Hda1 histone deacetylases facilitate repair of broken forks by promoting sister chromatid cohesion
(Nature Research, 2019)
Genome stability involves accurate replication and DNA repair. Broken replication forks, such as those encountering a nick, lead to double strand breaks (DSBs), which are preferentially repaired by sister-chromatid ...
Spontaneous DNA-RNA hybrids: differential impacts throughout the cell cycle
(Taylor & Francis, 2020)
A large body of research supports that transcription plays a major role among the many sources of replicative stress contributing to genome instability. It is therefore not surprising that the DNA damage response has a ...
Histone deacetylases facilitate the accurate repair of broken forks
(Taylor & Francis, 2020)
We have recently uncovered that loss of the yeast histone deacetylases Rpd3 (Reduced Potassium Dependency 3) and Hda1 (Histone DeAcetylase 3) affects the cohesion between sister chromatids thus impairing repair of DNA ...
The DNA damage response acts as a safeguardagainst harmful DNA–RNA hybrids ofdifferent origins
(EMBO Press, 2019-07-24)
Despite playing physiological roles in specific situations, DNA–RNA hybrids threat genome integrity. To investigate how cells do counteract spontaneous DNA–RNA hybrids, here we screen an siRNA library covering 240 human ...
Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
(Shared Science Publishers OG, 2019)
Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular ...
Histone Mutants Separate R Loop Formation from Genome Instability Induction
R loops have positive physiological roles, but they can also be deleterious by causing genome instability, and the mechanisms for this are unknown. Here we identified yeast histone H3 and H4 mutations that facilitate R ...