Now showing items 1-9 of 9
FANCD2 facilitates replication through common fragile sites
Common fragile sites (CFSs) are genomic regions that are unstable under conditions of replicative stress. Although the characteristics of CFSs that render them vulnerable to stress are mainly associated with replication, ...
Multiple signaling kinases target Mrc1 to prevent genomic instability triggered by transcription-replication conflicts
(Nature Publishing Group:, 2018)
Conflicts between replication and transcription machineries represent a major source of genomic instability and cells have evolved strategies to prevent such conflicts. However, little is known regarding how cells cope ...
Zim17/Tim15 links mitochondrial iron–sulfur cluster biosynthesis to nuclear genome stability
(Oxford University Press, 2011)
Genomic instability is related to a wide-range of human diseases. Here, we show that mitochondrial iron–sulfur cluster biosynthesis is important for the maintenance of nuclear genome stability in Saccharomyces cerevisiae. ...
Yra1-bound RNA–DNA hybrids cause orientation-independent transcription– replication collisions and telomere instability
(Cold Spring Harbor Laboratory Press, 2018)
R loops are an important source of genome instability, largely due to their negative impact on replication progression. Yra1/ALY is an abundant RNA-binding factor conserved from yeast to humans and required for mRNA export, ...
A Meiotic Checkpoint Alters Repair Partner Bias to Permit Inter-sister Repair of Persistent DSBs
(Elsevier B.V., 2019)
Accurate meiotic chromosome segregation critically depends on the formation of inter-homolog crossovers initiated by double-strand breaks (DSBs). Inaccuracies in this process can drive aneuploidy and developmental defects, ...
The DNA damage response acts as a safeguardagainst harmful DNA–RNA hybrids ofdifferent origins
(EMBO Press, 2019-07-24)
Despite playing physiological roles in specific situations, DNA–RNA hybrids threat genome integrity. To investigate how cells do counteract spontaneous DNA–RNA hybrids, here we screen an siRNA library covering 240 human ...
Histone Mutants Separate R Loop Formation from Genome Instability Induction
R loops have positive physiological roles, but they can also be deleterious by causing genome instability, and the mechanisms for this are unknown. Here we identified yeast histone H3 and H4 mutations that facilitate R ...
Harmful DNA:RNA hybrids are formed in cis and in a Rad51-independent manner
(eLife Sciences Publications, 2020)
DNA:RNA hybrids constitute a well-known source of recombinogenic DNA damage. The current literature is in agreement with DNA:RNA hybrids being produced co-transcriptionally by the invasion of the nascent RNA molecule ...