Now showing items 1-5 of 5
Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability
Although correlations between RNA polymerase II (RNAPII) transcription stress, R-loops, and genome instability have been established, the mechanisms underlying these connections remain poorly understood. Here, we used a ...
What causes an RNA-DNA hybrid to compromise genome integrity?
Transcription is a source of genome instability that stimulates mutation and recombination. Part of the damage produced by transcription is mediated by R-loops, non-B DNA structures that normally form by the re-annealing ...
Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
(Shared Science Publishers OG, 2019)
Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular ...
The antitumor drugs trabectedin and lurbinectedin induce transcription-dependent replication stress and genome instability
(American Association for Cancer Research, 2019)
R-loops are a major source of replication stress, DNA damage, and genome instability, which are major hallmarks of cancer cells. Accordingly, growing evidence suggests that R-loops may also be related to cancer. Here we ...
Harmful DNA:RNA hybrids are formed in cis and in a Rad51-independent manner
(eLife Sciences Publications, 2020)
DNA:RNA hybrids constitute a well-known source of recombinogenic DNA damage. The current literature is in agreement with DNA:RNA hybrids being produced co-transcriptionally by the invasion of the nascent RNA molecule ...