Repositorio de producción científica de la Universidad de Sevilla

A new role for Rrm3 in repair of replication-born DNA breakage by sister chromatid recombination

Opened Access A new role for Rrm3 in repair of replication-born DNA breakage by sister chromatid recombination

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Muñoz Galván, Sandra
García Rubio, María Luisa
Ortega, Pedro
Ruiz Pérez, José Francisco
Jimeno González, Sonia
Pardo, Benjamín
Gómez González, Belén María
Aguilera López, Andrés
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2017
Publicado en: PLoS Genetics, 13 (5), 1-18.
Tipo de documento: Artículo
Resumen: Replication forks stall at different DNA obstacles such as those originated by transcription. Fork stalling can lead to DNA double-strand breaks (DSBs) that will be preferentially repaired by homologous recombination when the sister chromatid is available. The Rrm3 helicase is a replisome component that promotes replication upon fork stalling, accumulates at highly transcribed regions and prevents not only transcription-induced replication fork stalling but also transcription-associated hyper-recombination. This led us to explore the possible role of Rrm3 in the repair of DSBs when originating at the passage of the replication fork. Using a mini-HO system that induces mainly single-stranded DNA breaks, we show that rrm3Δ cells are defective in DSB repair. The defect is clearly seen in sister chromatid recombination, the major repair pathway of replication-born DSBs. Our results indicate that Rrm3 recruitment to replication-born DSBs is crucial for viability, uncovering a new role for ...
[Ver más]
Cita: Muñoz Galván, S., García Rubio, M.L., Ortega, P., Ruiz Pérez, J.F., Jimeno González, S., Pardo, B.,...,Aguilera López, A. (2017). A new role for Rrm3 in repair of replication-born DNA breakage by sister chromatid recombination. PLoS Genetics, 13 (5), 1-18.
Tamaño: 6.483Mb
Formato: PDF

URI: http://hdl.handle.net/11441/61042

DOI: http://dx.doi.org/ 10.1371/journal.pgen.1006781

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones