Artículo
Development of cytosolic hypoxia and hypoxia-inducible factor stabilization are facilitated by aquaporin-1 expression
Autor/es | Echevarría Irusta, Miriam
![]() ![]() ![]() ![]() ![]() ![]() ![]() Muñoz Cabello, Ana María ![]() ![]() ![]() ![]() ![]() ![]() ![]() Sánchez Silva, Rocío Toledo Aral, Juan José ![]() ![]() ![]() ![]() ![]() ![]() ![]() López Barneo, José ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Departamento | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica |
Fecha de publicación | 2007 |
Fecha de depósito | 2024-02-13 |
Publicado en |
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Resumen | O2 is essential for aerobic life, and the classic view is that it diffuses freely across the plasma membrane. However, measurements of O 2 permeability of lipid bilayers have indicated that it is much lower than previously ... O2 is essential for aerobic life, and the classic view is that it diffuses freely across the plasma membrane. However, measurements of O 2 permeability of lipid bilayers have indicated that it is much lower than previously thought, and therefore, the existence of membrane O2 channels has been suggested. We hypothesized that, besides its role as a water channel, aquaporin-1 (AQP-1) could also work as an O2 transporter, because this transmembrane protein appears to be CO2-permeable and is highly expressed in cells with rapid O2 turnover (erythrocytes and microvessel endothelium). Here we show that in mammalian cells overexpressing AQP-1 and exposed to hypoxia, the loss of cytosolic O2, as well as stabilization of the O2-dependent hypoxia-inducible transcription factor and expression of its target genes, is accelerated. In normoxic endothelial cells, knocking down AQP-1 produces induction of hypoxia-inducible genes. Moreover, lung AQP-1 is markedly up-regulated in animals exposed to hypoxia. These data suggest that AQP-1 has O2 permeability and thus could facilitate O2 diffusion across the cell membrane. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc. |
Cita | Echevarría Irusta, M., Muñoz Cabello, A.M., Sánchez Silva, R., Toledo Aral, J.J. y López Barneo, J. (2007). Development of cytosolic hypoxia and hypoxia-inducible factor stabilization are facilitated by aquaporin-1 expression. Journal of Biological Chemistry, 282 (41), 30207-30215. https://doi.org/10.1074/jbc.M702639200. |
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