Artículo
Chronic Stress Alters the Expression Levels of Longevity-related Genes in the Rat Hippocampus
Autor/es | Sánchez Hidalgo, Ana C.
Muñoz Pinto, Mario Faustino Herrera Carmona, Antonio José Espinosa Oliva, Ana María Stowell, Rianne Ayala Gómez, Antonio Machado, Alberto Venero Recio, José Luis Martínez de Pablos, Rocío |
Departamento | Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica |
Fecha de publicación | 2016 |
Fecha de depósito | 2024-01-25 |
Publicado en |
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Resumen | The molecular mechanisms underlying the negative effects of psychological stress on cellular stress during aging and neurodegenerative diseases are poorly understood. The main objective of this study was to test the effect ... The molecular mechanisms underlying the negative effects of psychological stress on cellular stress during aging and neurodegenerative diseases are poorly understood. The main objective of this study was to test the effect of chronic psychological stress, and the consequent increase of circulating glucocorticoids, on several hippocampal genes involved in longevity. Sirtuin-1, p53, thioredoxin-interacting protein, and heat shock protein 70 were studied at the mRNA and protein levels in stressed and non-stressed animals. Stress treatment for 10 days decreased sirtuin-1 and heat shock protein 70 levels, but increased levels of p53, thioredoxin-interacting protein and the NADPH oxidase enzyme. Examination of protein expression following two months of stress treatment indicated that sirtuin-1 remained depressed. In contrast, an increase was observed for thioredoxin-interacting protein, heat shock protein 70, p53 and the NADPH oxidase enzyme. The effect of stress was reversed by mifepristone, a glucocorticoid receptor antagonist. These data suggest that chronic stress could contribute to aging in the hippocampus. |
Agencias financiadoras | Junta de Andalucía Ministerio de Economía y Competitividad (MINECO). España |
Identificador del proyecto | P09-CTS-5244
P10-CTS-6494 SAF2012-3902 |
Cita | Sánchez Hidalgo, A.C., Muñoz Pinto, M.F., Herrera Carmona, A.J., Espinosa Oliva, A.M., Stowell, R., Ayala Gómez, A.,...,Martínez de Pablos, R. (2016). Chronic Stress Alters the Expression Levels of Longevity-related Genes in the Rat Hippocampus. Neurochemistry International, 97, 181-192. https://doi.org/10.1016/j.neuint.2016.04.009. |
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