Simvastatin and metformin inhibit cell growth in hepatitis C virus infected cells via mTOR increasing PTEN and autophagy
|Author||Del Campo, Jose A.
García Valdecasas, Marta
Gil Gómez, Antonio
Rojas Alvarez-Ossorio, M. Angeles
Ampuero Herrojo, Javier
Gallego Durán, Rocío
Padillo Ruiz, Francisco Javier
Muntané Relat, Jordi
Romero Gómez, Manuel
|Department||Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica
Universidad de Sevilla. Departamento de Cirugía
|Published in||PLoS ONE, 13 (1)|
|Abstract||Hepatitis C virus (HCV) infection has been related to increased risk of development of hepa tocellular carcinoma (HCC) while metformin (M) and statins treatment seemed to protect
against HCC development. In this work, we ...
Hepatitis C virus (HCV) infection has been related to increased risk of development of hepa tocellular carcinoma (HCC) while metformin (M) and statins treatment seemed to protect against HCC development. In this work, we aim to identify the mechanisms by which metfor min and simvastatin (S) could protect from liver cancer. Huh7.5 cells were infected with HCV particles and treated with M+S. Human primary hepatocytes were treated with M+S. Treatment with both drugs inhibited Huh7.5 cell growth and HCV infection. In non-infected cells S increased translational controlled tumor protein (TCTP) and phosphatase and tensin homolog (PTEN) proteins while M inhibited mammalian target of rapamycin (mTOR) and TCTP. Simvastatin and metformin co-administered down-regulated mTOR and TCTP, while PTEN was increased. In cells infected by HCV, mTOR, TCTP, p62 and light chain 3B II (LC3BII) were increased and PTEN was decreased. S+M treatment increased PTEN, p62 and LC3BII in Huh7.5 cells. In human primary hepatocytes, metformin treatment inhibited mTOR and PTEN, but up-regulated p62, LC3BII and Caspase 3. In conclusion, simvastatin and metformin inhibited cell growth and HCV infection in vitro. In human hepatocytes, met formin increased cell-death markers. These findings suggest that M+S treatment could be useful in therapeutic prevention of HCV-related hepatocellular carcinoma.
|Cite||Del Campo, J.A., García Valdecasas, M., Gil Gómez, A., Rojas Alvarez-Ossorio, M.A., Gallego, P., Ampuero Herrojo, J.,...,Romero Gómez, M. (2018). Simvastatin and metformin inhibit cell growth in hepatitis C virus infected cells via mTOR increasing PTEN and autophagy. PLoS ONE, 13 (1)|