Repositorio de producción científica de la Universidad de Sevilla

TDP2 suppresses chromosomal translocations induced by DNA topoisomerase II during gene transcription

Opened Access TDP2 suppresses chromosomal translocations induced by DNA topoisomerase II during gene transcription

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Gómez Herreros, Fernando
Zagnoli Vieira, Guido
Ntai, Ioanna
Martínez Macías, María Isabel
Anderson, Rhona M.
Herrero Ruíz, Andrés
Caldecott, Keith W.
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2017
Publicado en: Nature Communications, 8 (233), 1-8.
Tipo de documento: Artículo
Resumen: DNA double-strand breaks (DSBs) induced by abortive topoisomerase II (TOP2) activity are a potential source of genome instability and chromosome translocation. TOP2-induced DNA double-strand breaks are rejoined in part by tyrosyl-DNA phosphodiesterase 2 (TDP2)-dependent non-homologous end-joining (NHEJ), but whether this process suppresses or promotes TOP2-induced translocations is unclear. Here, we show that TDP2 rejoins DSBs induced during transcription-dependent TOP2 activity in breast cancer cells and at the translocation ‘hotspot’, MLL. Moreover, we find that TDP2 suppresses chromosome rearrangements induced by TOP2 and reduces TOP2-induced chromosome translocations that arise during gene transcription. Interestingly, however, we implicate TDP2-dependent NHEJ in the formation of a rare subclass of translocations associated previously with therapy-related leukemia and characterized by junction sequences with 4-bp of perfect homology. Collectively, these data highlight the threat p...
[Ver más]
Cita: Gómez Herreros, F., Zagnoli Vieira, G., Ntai, I., Martínez Macías, M.I., Anderson, R.M., Herrero Ruíz, A. y Caldecott, .W. (2017). TDP2 suppresses chromosomal translocations induced by DNA topoisomerase II during gene transcription. Nature Communications, 8 (233), 1-8.
Tamaño: 1.050Mb
Formato: PDF

URI: http://hdl.handle.net/11441/63912

DOI: 10.1038/s41467-017-00307-y

Ver versión del editor

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones