dc.creator | Pérez Calero, Carmen | es |
dc.creator | Bayona Feliu, Aleix | es |
dc.creator | Xue, Xiaoyu | es |
dc.creator | Barroso Ceballos, Sonia Inés | es |
dc.creator | Muñoz Sánchez, Sergio | es |
dc.creator | González Basallote, Víctor M. | es |
dc.creator | Sung, Patrick | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2020-06-25T16:44:43Z | |
dc.date.available | 2020-06-25T16:44:43Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Pérez Calero, C., Bayona Feliu, A., Xue, X., Barroso Ceballos, S.I., Muñoz Sánchez, S., González Basallote, V.M.,...,Aguilera López, A. (2020). UAP56/DDX39B is a major cotranscriptional RNA–DNA helicase that unwinds harmful R loops genome-wide. Genes & Development, 34, 1-15. | |
dc.identifier.issn | 0890-9369 | es |
dc.identifier.issn | 1549-5477 | es |
dc.identifier.uri | https://hdl.handle.net/11441/98271 | |
dc.description.abstract | Nonscheduled R loops represent a major source of DNA damage and replication stress. Cells have different ways to prevent R-loop accumulation. One mechanism relies on the conserved THO complex in association with cotranscriptional RNA processing factors including the RNA-dependent ATPase UAP56/DDX39B and histone modifiers such as the SIN3 deacetylase in humans. We investigated the function of UAP56/DDX39B in R-loop removal. We show that UAP56 depletion causes R-loop accumulation, R-loop-mediated genome instability, and replication fork stalling. We demonstrate an RNA–DNA helicase activity in UAP56 and show that its overexpression suppresses R loops and genome instability induced by depleting five different unrelated factors. UAP56/DDX39B localizes to active chromatin and prevents the accumulation of RNA–DNA hybrids over the entire genome. We propose that, in addition to its RNA processing role, UAP56/DDX39B is a key helicase required to eliminate harmful cotranscriptional RNA structures that otherwise would block transcription and replication. | es |
dc.description.sponsorship | European Research Council ERC2014 AdG669898 TARLOOP | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BFU2013-42918-P, BFU2016- 75058-P | es |
dc.format | application/pdf | es |
dc.format.extent | 16 p. | es |
dc.language.iso | eng | es |
dc.publisher | Cold Spring Harbor Laboratory Press | es |
dc.relation.ispartof | Genes & Development, 34, 1-15. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Double-strand breaks | es |
dc.subject | Genome instability | es |
dc.subject | R loops | es |
dc.subject | RNA–DNA helicase | es |
dc.subject | RNA–DNA hybrids | es |
dc.subject | Replication fork stalling | es |
dc.subject | UAP56/DDX39B | es |
dc.title | UAP56/DDX39B is a major cotranscriptional RNA–DNA helicase that unwinds harmful R loops genome-wide | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | ERC2014 AdG669898 TARLOOP | es |
dc.relation.projectID | BFU2013-42918-P | es |
dc.relation.projectID | BFU2016- 75058-P | es |
dc.relation.publisherversion | https://doi.org/10.1101/gad.336024.119 | es |
dc.identifier.doi | 10.1101/gad.336024.119 | es |
dc.journaltitle | Genes & Development | es |
dc.publication.volumen | 34 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 15 | es |