Article
The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes
Author/s | Olombrada, Miriam
Pena, Cohue Rodríguez Galán, Olga Klingauf Nerurkar, Purnima Portugal Calisto, Daniela Oborska Oplová, Michaela Altvater, Martín Gavilanes, Jose G. Martínez del Pozo, Álvaro Cruz Díaz, Jesús de la ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Department | Universidad de Sevilla. Departamento de Genética |
Publication Date | 2020 |
Deposit Date | 2020-05-05 |
Published in |
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Abstract | The ribotoxin α-sarcin belongs to a family of ribonucleases that cleave the sarcin/ricin loop (SRL), a critical functional rRNA element within the large ribosomal subunit (60S), thereby abolishing translation. Whether ... The ribotoxin α-sarcin belongs to a family of ribonucleases that cleave the sarcin/ricin loop (SRL), a critical functional rRNA element within the large ribosomal subunit (60S), thereby abolishing translation. Whether α-sarcin targets the SRL only in mature 60S subunits remains unresolved. Here, we show that, in yeast, α-sarcin can cleave SRLs within late 60S pre-ribosomes containing mature 25S rRNA but not nucleolar/nuclear 60S pre-ribosomes containing 27S pre-rRNA in vivo. Conditional expression of α-sarcin is lethal, but does not impede early pre-rRNA processing, nuclear export and the cytoplasmic maturation of 60S pre-ribosomes. Thus, SRL-cleaved containing late 60S pre-ribosomes seem to escape cytoplasmic proofreading steps. Polysome analyses revealed that SRL-cleaved 60S ribosomal subunits form 80S initiation complexes, but fail to progress to the step of translation elongation. We suggest that the functional integrity of a α-sarcin cleaved SRL might be assessed only during translation. |
Funding agencies | Ministerio de Economía y Competitividad (MINECO). España European union ERFD |
Project ID. | program [BFU2012-32404]
![]() [BFU2016-75352-P] ![]() |
Citation | Olombrada, M., Pena, C., Rodríguez Galán, O., Klingauf Nerurkar, P., Portugal Calisto, D., Oborska Oplová, M.,...,Cruz Díaz, J.d.l. (2020). The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes. Nucleic Acids Research, 48 (11), 6210-6222. |
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