dc.creator | Olombrada, Miriam | es |
dc.creator | Pena, Cohue | es |
dc.creator | Rodríguez Galán, Olga | es |
dc.creator | Klingauf Nerurkar, Purnima | es |
dc.creator | Portugal Calisto, Daniela | es |
dc.creator | Oborska Oplová, Michaela | es |
dc.creator | Altvater, Martín | es |
dc.creator | Gavilanes, Jose G. | es |
dc.creator | Martínez del Pozo, Álvaro | es |
dc.creator | Cruz Díaz, Jesús de la | es |
dc.date.accessioned | 2020-05-05T08:36:04Z | |
dc.date.available | 2020-05-05T08:36:04Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Olombrada, M., Pena, C., Rodríguez Galán, O., Klingauf Nerurkar, P., Portugal Calisto, D., Oborska Oplová, M.,...,Cruz Díaz, J.d.l. (2020). The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes. Nucleic Acids Research, 48 (11), 6210-6222. | |
dc.identifier.issn | 1362-4962 | es |
dc.identifier.uri | https://hdl.handle.net/11441/96123 | |
dc.description.abstract | The ribotoxin α-sarcin belongs to a family of ribonucleases that cleave the sarcin/ricin loop (SRL), a critical functional rRNA element within the large ribosomal subunit (60S), thereby abolishing translation. Whether α-sarcin targets the SRL only in mature 60S subunits remains unresolved. Here, we show that, in yeast, α-sarcin can cleave SRLs within late 60S pre-ribosomes containing mature 25S rRNA but not nucleolar/nuclear 60S pre-ribosomes containing 27S pre-rRNA in vivo. Conditional expression of α-sarcin is lethal, but does not impede early pre-rRNA processing, nuclear export and the cytoplasmic maturation of 60S pre-ribosomes. Thus, SRL-cleaved containing late 60S pre-ribosomes seem to escape cytoplasmic proofreading steps. Polysome analyses revealed that SRL-cleaved 60S ribosomal subunits form 80S initiation complexes, but fail to progress to the step of translation elongation. We suggest that the functional integrity of a α-sarcin cleaved SRL might be assessed only during translation. | es |
dc.description.sponsorship | Spanish Ministry of Economy and Competitiveness MINECO and the European Union ERFD program [BFU2012-32404] to A.M.P., [BFU2016-75352-P] | es |
dc.format | application/pdf | es |
dc.format.extent | 13 p. | es |
dc.language.iso | eng | es |
dc.publisher | Oxford University Press | es |
dc.relation.ispartof | Nucleic Acids Research, 48 (11), 6210-6222. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | program [BFU2012-32404] | es |
dc.relation.projectID | [BFU2016-75352-P] | es |
dc.relation.publisherversion | https://doi.org/10.1093/nar/gkaa315 | es |
dc.identifier.doi | 10.1093/nar/gkaa315 | es |
dc.journaltitle | Nucleic Acids Research | es |
dc.publication.volumen | 48 | |
dc.publication.issue | 11 | |
dc.publication.initialPage | 6210 | es |
dc.publication.endPage | 6222 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | es |
dc.contributor.funder | European union ERFD | es |