Mostrar el registro sencillo del ítem

Artículo

dc.creatorHinojosa Hidalgo, María Graciaes
dc.creatorPrieto Ortega, Ana Isabeles
dc.creatorGutiérrez-Praena, Danieles
dc.creatorMoreno Onorato, Francisco Javieres
dc.creatorCameán Fernández, Ana Maríaes
dc.creatorJos Gallego, Ángeles Mencíaes
dc.date.accessioned2020-01-21T12:58:10Z
dc.date.available2020-01-21T12:58:10Z
dc.date.issued2019-02-27
dc.identifier.citationHinojosa Hidalgo, M.G., Prieto Ortega, A.I., Gutiérrez-Praena, D., Moreno Onorato, F.J., Cameán Fernández, A.M. y Jos Gallego, Á.M. (2019). Neurotoxic assessment of Microcystin-LR, cylindrospermopsin andtheir combination on the human neuroblastoma SH-SY5Y cell line. Chemosphere, 224, 751-764.
dc.identifier.issn0045-6535es
dc.identifier.urihttps://hdl.handle.net/11441/92013
dc.description.abstractMicrocystin-LR (MC-LR) and Cylindrospermopsin (CYN) are produced by cyanobacteria. Although being considered as a hepatotoxin and a cytotoxin, respectively, different studies have revealed neurotoxic properties for both of them. The aim of the present work was to study their cytotoxic effects, alone and in combination, in the SH-SY5Y cell line. In addition, toxicity mechanisms such as oxidative stress and acetylcholinesterase (AChE) activity, and morphological studies were carried out. Results showed a cytotoxic response of the cells after their exposure to 0–100 μg/mL of MC-LR or 0–10 μg/mL CYN in both differentiated and undifferentiated cells. Thus, CYN resulted to be more toxic than MC-LR. Respect to their combination, a higher cytotoxic effect than the toxins alone in the case of undifferentiated cells, and almost a similar response to the presented by MC-LR in differentiated cells were observed. However, after analyzing this data with the isobolograms method, an antagonistic effect was mainly obtained. The oxidative stress study only showed an affectation of glutathione levels at the highest concentrations assayed of MC-LR and the combination in the undifferentiated cells. A significant increase in the AChE activity was observed after exposure to MC-LR in undifferentiated cells, and after exposure to the combination of both cyanotoxins on differentiated cells. However, CYN decreased the AChE activity only on differentiated cultures. Finally, the morphological study revealed different signs of cellular affectation, with apoptotic processes at all the concentrations assayed. Therefore, both cyanotoxins isolated and in combination, have demonstrated to cause neurotoxic effects in the SH-SY5Y cell line.es
dc.description.sponsorshipMinisterio de Economía y Com-petitividad of Spain (AGL2015-64558-R, MINECO/FEDER, UE)es
dc.description.sponsorshipJunta de Andalucia for the contact of María Gracia Hinojosa (USE-16667)es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofChemosphere, 224, 751-764.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMC-LRes
dc.subjectCYNes
dc.subjectMC-LR+CYN combinationes
dc.subjectSH-SY5Y cellses
dc.subjectNeurotoxicityes
dc.titleNeurotoxic assessment of Microcystin-LR, cylindrospermopsin andtheir combination on the human neuroblastoma SH-SY5Y cell linees
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legales
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Celulares
dc.relation.projectIDAGL2015-64558-Res
dc.relation.projectIDUSE-16667es
dc.relation.publisherversionhttps://doi.org/10.1016/j.chemosphere.2019.02.173es
dc.identifier.doi10.1016/j.chemosphere.2019.02.173es
idus.format.extent14 p.es
dc.journaltitleChemospherees
dc.publication.volumen224es
dc.publication.initialPage751es
dc.publication.endPage764es
dc.description.awardwinningPremio Mensual Publicación Científica Destacada de la US. Facultad de Farmacia

FicherosTamañoFormatoVerDescripción
10.1016_j.chemosphere.2019.02. ...9.374MbIcon   [PDF] Ver/Abrir   Postprint

Este registro aparece en las siguientes colecciones

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como: Attribution-NonCommercial-NoDerivatives 4.0 Internacional