The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation
|Author/s||Gómez Herreros, Fernando
Rodríguez Galán, Olga
Begley, Victoria Sarah
Millán Zambrano, Gonzalo
Morillo Huesca, Macarena
Muñoz Centeno, María de la Cruz
Pérez Ortín, José Enrique
Cruz Díaz, Jesús de la
Holstege, Frank C.P.
Chávez de Diego, Sebastián
|Department||Universidad de Sevilla. Departamento de Genética|
|Abstract||Ribosome assembly requires the concerted expression of hundreds of genes, which are transcribed by all three nuclear RNA polymerases. Transcription elongation involves dynamic interactions between RNA polymerases and ...
Ribosome assembly requires the concerted expression of hundreds of genes, which are transcribed by all three nuclear RNA polymerases. Transcription elongation involves dynamic interactions between RNA polymerases and chromatin. We performed a synthetic lethal screening in Saccharomyces cerevisiae with a conditional allele of SPT6, which encodes one of the factors that facilitates this process. Some of these synthetic mutants corresponded to factors that facilitate pre-rRNA processing and ribosome biogenesis. We found that the in vivo depletion of one of these factors, Arb1, activated transcription elongation in the set of genes involved directly in ribosome assembly. Under these depletion conditions, Spt6 was physically targeted to the upregulated genes, where it helped maintain their chromatin integrity and the synthesis of properly stable mRNAs. The mRNA profiles of a large set of ribosome biogenesismutants confirmed the existence of a feedback regulatory network among ribosome assembly genes. The transcriptional response in this network depended on both the specific malfunction and the role of the regulated gene. In accordance with our screening, Spt6 positively contributed to the optimal operation of this global network. On the whole, this work uncovers a feedback control of ribosome biogenesis by fine-tuning transcription elongation in ribosome assembly factor-coding genes.
|Citation||Gómez Herreros, F., Margaritis, T., Rodríguez Galán, O., Pelechano, V., Begley, V.S., Millán Zambrano, G.,...,Chávez de Diego, S. (2017). The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation. Nucleic Acids Research, 45 (16), 9302-9318.|