Artículo
The Rad50 coiled-coil domain is indispensable for Mre11 complex functions
Autor/es | Hohl, Marcel
Muñoz Galván, Sandra Tous Rivera, Cristina Aguilera López, Andrés Petrini, John H. J. |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2011 |
Fecha de depósito | 2018-03-19 |
Publicado en |
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Resumen | The Mre11 complex (Mre11, Rad50 and Xrs2 in Saccharomyces cerevisiae) influences diverse functions in the DNA damage response. The complex comprises the globular DNA-binding domain and the Rad50 hook domain, which are ... The Mre11 complex (Mre11, Rad50 and Xrs2 in Saccharomyces cerevisiae) influences diverse functions in the DNA damage response. The complex comprises the globular DNA-binding domain and the Rad50 hook domain, which are linked by a long and extended Rad50 coiled-coil domain. In this study, we constructed rad50 alleles encoding truncations of the coiled-coil domain to determine which Mre11 complex functions required the full length of the coils. These mutations abolished telomere maintenance and meiotic double-strand break (DSB) formation, and severely impaired homologous recombination, indicating a requirement for long-range action. Nonhomologous end joining, which is probably mediated by the globular domain of the Mre11 complex, was also severely impaired by alteration of the coiled-coil and hook domains, providing the first evidence of their influence on this process. These data show that functions of Mre11 complex are integrated by the coiled coils of Rad50. |
Agencias financiadoras | Swiss National Science Foundation (SNFS) Ministerio de Ciencia e Innovación (MICIN). España |
Identificador del proyecto | GM56888
PBZH33-112756 PA0033-117484 BFU2006-05260 2010 CSD2007-015 |
Cita | Hohl, M., Muñoz Galván, S., Tous Rivera, C., Aguilera López, A. y Petrini, J.H.J. (2011). The Rad50 coiled-coil domain is indispensable for Mre11 complex functions. Nature Structural and Molecular Biology, 18 (10), 1124-1131. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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The Rad50 Coiled Coil Domain.pdf | 3.319Mb | [PDF] | Ver/ | |