Artículo
Histone variant H2A.Z is needed for efficient transcription-coupled NER and genome integrity in UV challenged yeast cells
Autor/es | Gaillard, Hélène
Ciudad, Toni Aguilera López, Andrés Wellinger, Ralf Erik |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2024-09-10 |
Fecha de depósito | 2024-09-25 |
Publicado en |
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Resumen | The genome of living cells is constantly challenged by DNA lesions that interfere with cellular processes such as transcription and replication. A manifold of mechanisms act in concert to ensure adequate DNA repair, gene ... The genome of living cells is constantly challenged by DNA lesions that interfere with cellular processes such as transcription and replication. A manifold of mechanisms act in concert to ensure adequate DNA repair, gene expression, and genome stability. Bulky DNA lesions, such as those induced by UV light or the DNA-damaging agent 4-nitroquinoline oxide, act as transcriptional and replicational roadblocks and thus represent a major threat to cell metabolism. When located on the transcribed strand of active genes, these lesions are handled by transcription-coupled nucleotide excision repair (TC-NER), a yet incompletely understood NER sub-pathway. Here, using a genetic screen in the yeast Saccharomyces cerevisiae, we identified histone variant H2A.Z as an important component to safeguard transcription and DNA integrity following UV irradiation. In the absence of H2A.Z, repair by TC-NER is severely impaired and RNA polymerase II clearance reduced, leading to an increase in double-strand breaks. Thus, H2A.Z is needed for proficient TC-NER and plays a major role in the maintenance of genome stability upon UV irradiation. |
Agencias financiadoras | Ministerio de Ciencia, Innovación y Universidades (MICINN). España Junta de Andalucía Universidad de Sevilla Ministerio de Economía y Competitividad (MINECO). España |
Identificador del proyecto | PID2022-140466NB-I00
BFU2016-75058-P P20_01220 PP2018-10767 PP2019- 13299 BFU2013-42918-P |
Cita | Gaillard, H., Ciudad, T., Aguilera López, A. y Wellinger, R.E. (2024). Histone variant H2A.Z is needed for efficient transcription-coupled NER and genome integrity in UV challenged yeast cells. PLoS Genetics, 20 (9), e1011300. https://doi.org/10.1371/journal.pgen.1011300. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Histone variant.pdf | 2.931Mb | [PDF] | Ver/ | Versión publicada |