dc.creator | Williams, Jonathan D. | es |
dc.creator | Zhu, Demi | es |
dc.creator | García Rubio, María Luisa | es |
dc.creator | Shaltz, Samantha | es |
dc.creator | Aguilera López, Andrés | es |
dc.creator | Jinks-Robertson, Sue | es |
dc.date.accessioned | 2024-09-12T14:59:42Z | |
dc.date.available | 2024-09-12T14:59:42Z | |
dc.date.issued | 2023-06 | |
dc.identifier.citation | Williams, J.D., Zhu, D., García Rubio, M.L., Shaltz, S., Aguilera López, A. y Jinks-Robertson, S. (2023). Spontaneous deamination of cytosine to uracil is biased to the non-transcribed DNA strand in yeast. DNA Repair, 126, 103489. https://doi.org/10.1016/j.dnarep.2023.103489. | |
dc.identifier.issn | 1568-7856 | es |
dc.identifier.issn | 1568-7864 | es |
dc.identifier.uri | https://hdl.handle.net/11441/162455 | |
dc.description.abstract | Transcription in Saccharomyces cerevisiae is associated with elevated mutation and this partially reflects enhanced damage of the corresponding DNA. Spontaneous deamination of cytosine to uracil leads to CG>TA mutations that provide a strand-specific read-out of damage in strains that lack the ability to remove uracil from DNA. Using the CAN1 forward mutation reporter, we found that C>T and G>A mutations, which reflect deamination of the non-transcribed and transcribed DNA strands, respectively, occurred at similar rates under low-transcription conditions. By contrast, the rate of C>T mutations was 3-fold higher than G>A mutations under high-transcription conditions, demonstrating biased deamination of the non-transcribed strand (NTS). The NTS is transiently single-stranded within the ∼15 bp transcription bubble, or a more extensive region of the NTS can be exposed as part of an R-loop that can form behind RNA polymerase. Neither the deletion of genes whose products restrain R-loop formation nor the over-expression of RNase H1, which degrades R-loops, reduced the biased deamination of the NTS, and no transcription-associated R-loop formation at CAN1 was detected. These results suggest that the NTS within the transcription bubble is a target for spontaneous deamination and likely other types of DNA damage. | es |
dc.description.sponsorship | National Institutes of Health R35GM118077 | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2019-104270GB-I00/BMC | es |
dc.format | application/pdf | es |
dc.format.extent | 21 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | DNA Repair, 126, 103489. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Transcription | es |
dc.subject | Mutagenesis | es |
dc.subject | RNA polymerase | es |
dc.subject | R-loops | es |
dc.subject | Transcription bubble | es |
dc.subject | Deamination | es |
dc.title | Spontaneous deamination of cytosine to uracil is biased to the non-transcribed DNA strand in yeast | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | R35GM118077 | es |
dc.relation.projectID | PID2019-104270GB-I00/BMC | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.dnarep.2023.103489 | es |
dc.identifier.doi | 10.1016/j.dnarep.2023.103489 | es |
dc.journaltitle | DNA Repair | es |
dc.publication.volumen | 126 | es |
dc.publication.initialPage | 103489 | es |
dc.contributor.funder | National Institutes of Health. United States | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |