Article
Chemical and biological characterization of the DPP-IV inhibitory activity exerted by lupin (Lupinus angustifolius) peptides: From the bench to the bedside investigation
Author/s | Cruz-Chamorro, Ivan
Santos-Sanchez, Guillermo Bollati, Carlotta Bartolomei, Martina Capriotti, Anna Laura Cerrato, Andrea Millán-Linares, María del Carmen Carrillo Vico, Antonio Lammi, Carmen |
Department | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología Instituto de Biomedicina de Sevilla (IBIS) |
Publication Date | 2023 |
Deposit Date | 2024-05-17 |
Published in |
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Awards | Premio Mensual Publicación Científica Destacada de la US. Facultad de Medicina |
Abstract | Dipeptidyl peptidase IV (DPP-IV) is considered a key target for the diabetes treatment, since it is involved in glucose metabolism. Although lupin protein consumption shown hypoglycemic activity, there is no evidence of ... Dipeptidyl peptidase IV (DPP-IV) is considered a key target for the diabetes treatment, since it is involved in glucose metabolism. Although lupin protein consumption shown hypoglycemic activity, there is no evidence of its effect on DPP-IV activity. This study demonstrates that a lupin protein hydrolysate (LPH), obtained by hydrolysis with Alcalase, exerts anti-diabetic activity by modulating DPP-IV activity. In fact, LPH decreased DPP-IV activity in a cell-free and cell-based system. Contextually, Caco-2 cells were employed to identify LPH peptides that can be intestinally trans-epithelial transported. Notably, 141 different intestinally transported LPH sequences were identified using nano- and ultra-chromatography coupled to mass spectrometry. Hence, it was demonstrated that LPH modulated the glycemic response and the glucose concentration in mice, by inhibiting the DPP-IV. Finally, a beverage containing 1 g of LPH decreased DPP-IV activity and glucose levels in humans. |
Funding agencies | Consejeria de Salud Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades, Junta de Andalucia Erasmus+ Mobility Programme Ministerio de Economia y Competitividad, Gobierno de Espana Ministerio de Educacion, Cultura y Deporte, Gobierno de Espana PAIDI Program from Junta de Andalucia Universidad de Sevilla |
Project ID. | PC-0111-2016-0111
VIPPIT-2020-II.4 AGL2012-40247-C02-01 AGL2012-40247-C02-02 FPU13/01210 DOC_00587/2020 CTS160 FPU16/02339 |
Citation | Cruz-Chamorro, I., Santos-Sanchez, G., Bollati, C., Bartolomei, M., Capriotti, A.L., Cerrato, A.,...,Lammi, C. (2023). Chemical and biological characterization of the DPP-IV inhibitory activity exerted by lupin (Lupinus angustifolius) peptides: From the bench to the bedside investigation. Food Chemistry, 426 (Volumen: 426 Número de artículo: 136458). https://doi.org/10.1016/j.foodchem.2023.136458. |
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