Article
Study of Zalema Grape Pomace: Phenolic Composition and Biological Effects in Caenorhabditis Elegans
Author/s | Jara Palacios, Mª José
González Manzano, Susana Escudero Gilete, María Luisa Hernanz Vila, María Dolores Dueñas, Montserrat González Paramás, Ana M. Heredia Mira, Francisco José Santos Buelga, Celestino |
Department | Universidad de Sevilla. Departamento de Química Analítica Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal |
Publication Date | 2013 |
Deposit Date | 2024-01-26 |
Published in |
|
Abstract | The phenolic composition of the extractable fraction of Zalema grape pomace has been analyzed by HPLC-DAD-MS and consisted of mainly flavanols and flavonols (122.75 and 23.11 mg/100 g dry pomace, respectively). The antioxidant ... The phenolic composition of the extractable fraction of Zalema grape pomace has been analyzed by HPLC-DAD-MS and consisted of mainly flavanols and flavonols (122.75 and 23.11 mg/100 g dry pomace, respectively). The antioxidant activity has been determined by in vitro FRAP, ABTS, and ORAC assays (11.7, 34.9, and 63.6 mmol of Trolox equivalents (TE) per 100 g of dry pomace, respectively) and in vivo using the model organism Caenorhabditis elegans. Cultivation of C. elegans in media containing 100 μg/mL dry pomace extract increased the survival of worms submitted to thermally induced oxidative stress, whereas a decrease in the rate of worm survival was found for 300 μg/mL extract. Interestingly, the levels of reactive oxygen species (ROS) were significantly decreased in stressed worms treated with the pomace extract at the two concentration levels. Further studies are required to explain this unexpected behavior, as well as to determine the compounds and mechanisms involved in the observed effects. |
Funding agencies | Gobierno de España |
Project ID. | AGL2009-12001
BFU2012-35228/BFI CSD2007-00063 |
Citation | Jara Palacios, M.J., González Manzano, S., Escudero Gilete, M.L., Hernanz Vila, M.D., Dueñas, M., González Paramás, A.M.,...,Santos Buelga, C. (2013). Study of Zalema Grape Pomace: Phenolic Composition and Biological Effects in Caenorhabditis Elegans. Journal of Agricultural and Food Chemistry, 61 (21), 5114-5121. https://doi.org/10.1021/jf400795s. |
Files | Size | Format | View | Description |
---|---|---|---|---|
Study of Zalema.pdf | 709.6Kb | [PDF] | View/ | Versión aceptada |
This item appears in the following collection(s)
This document is protected by intellectual and industrial property rights. Without prejudice to existing legal exemptions, its reproduction, distribution, public communication or transformation is prohibited without the authorization of the rights holder, unless otherwise indicated.