Artículo
Phosphorylation disrupts long-distance electron transport in cytochrome c
Autor/es | Gomila, Alexandre M.J.
Pérez Mejías, Gonzalo Nin-Hill, Alba Guerra Castellano, Alejandra Casas Ferrer, Laura Ortiz Tescari, Sthefany Díaz Quintana, Antonio Jesús Samitier, Josep Rovira, Carme Rosa Acosta, Miguel Ángel de la Díaz Moreno, Irene Gorostiza, Pau Giannotti, Marina I. Lagunas, Anna |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2022 |
Fecha de depósito | 2022-12-07 |
Publicado en |
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Resumen | t has been recently shown that electron transfer between mitochondrial cytochrome c and the cytochrome c1 subunit of the cytochrome bc1 can proceed at long-distance through the aqueous solution. Cytochrome c is thought to ... t has been recently shown that electron transfer between mitochondrial cytochrome c and the cytochrome c1 subunit of the cytochrome bc1 can proceed at long-distance through the aqueous solution. Cytochrome c is thought to adjust its activity by changing the affinity for its partners via Tyr48 phosphorylation, but it is unknown how it impacts the nanoscopic environment, interaction forces, and long-range electron transfer. Here, we constrain the orientation and separation between cytochrome c1 and cytochrome c or the phosphomimetic Y48pCMF cytochrome c, and deploy an array of single-molecule, bulk, and computational methods to investigate the molecular mechanism of electron transfer regulation by cytochrome c phosphorylation. We demonstrate that phosphorylation impairs long-range electron transfer, shortens the long-distance charge conduit between the partners, strengthens their interaction, and departs it from equilibrium. These results unveil a nanoscopic view of the interaction between redox protein partners in electron transport chains and its mechanisms of regulation. |
Agencias financiadoras | European Union (UE) Ministerio de Ciencia e Innovación (MICIN). España Generalitat de Catalunya Junta de Andalucía |
Identificador del proyecto | 945539
ICT-36-2020-101016787 PID2021-126663NB-I00 PID2019-111493RB-I00 PGC2018-096049-B-I00 CTQ2015-66194-R PID2020-118893GB-I00 2017-SGR-1079 2017-SGR-1442 2017-SGR-1189 US-1254317 US-1257019 P18-FR-3487 P18-HO-4091 |
Cita | Gomila, A.M.J., Pérez Mejías, G., Nin-Hill, A., Guerra Castellano, A., Casas Ferrer, L., Ortiz Tescari, S.,...,Lagunas, A. (2022). Phosphorylation disrupts long-distance electron transport in cytochrome c. Nature Communications, 13 (1), 7100. https://doi.org/10.1038/s41467-022-34809-1. |
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Phosphorylation disrupts.pdf | 3.338Mb | [PDF] | Ver/ | |