dc.creator | Gomila, Alexandre M.J. | es |
dc.creator | Pérez Mejías, Gonzalo | es |
dc.creator | Nin-Hill, Alba | es |
dc.creator | Guerra Castellano, Alejandra | es |
dc.creator | Casas Ferrer, Laura | es |
dc.creator | Ortiz Tescari, Sthefany | es |
dc.creator | Díaz Quintana, Antonio Jesús | es |
dc.creator | Samitier, Josep | es |
dc.creator | Rovira, Carme | es |
dc.creator | Rosa Acosta, Miguel Ángel de la | es |
dc.creator | Díaz Moreno, Irene | es |
dc.creator | Gorostiza, Pau | es |
dc.creator | Giannotti, Marina I. | es |
dc.creator | Lagunas, Anna | es |
dc.date.accessioned | 2022-12-07T18:50:49Z | |
dc.date.available | 2022-12-07T18:50:49Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Gomila, A.M.J., Pérez Mejías, G., Nin-Hill, A., Guerra Castellano, A., Casas Ferrer, L., Ortiz Tescari, S.,...,Lagunas, A. (2022). Phosphorylation disrupts long-distance electron transport in cytochrome c. Nature Communications, 13 (1), 7100. https://doi.org/10.1038/s41467-022-34809-1. | |
dc.identifier.issn | 2041-1723 | es |
dc.identifier.uri | https://hdl.handle.net/11441/140233 | |
dc.description.abstract | t has been recently shown that electron transfer between mitochondrial cytochrome c and the cytochrome c1 subunit of the cytochrome bc1 can proceed at long-distance through the aqueous solution. Cytochrome c is thought to adjust its activity by changing the affinity for its partners via Tyr48 phosphorylation, but it is unknown how it impacts the nanoscopic environment, interaction forces, and long-range electron transfer. Here, we constrain the orientation and separation between cytochrome c1 and cytochrome c or the phosphomimetic Y48pCMF cytochrome c, and deploy an array of single-molecule, bulk, and computational methods to investigate the molecular mechanism of electron transfer regulation by cytochrome c phosphorylation. We demonstrate that phosphorylation impairs long-range electron transfer, shortens the long-distance charge conduit between the partners, strengthens their interaction, and departs it from equilibrium. These results unveil a nanoscopic view of the interaction between redox protein partners in electron transport chains and its mechanisms of regulation. | es |
dc.description.sponsorship | European Union 945539, ICT-36-2020-101016787 | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2021-126663NB-I00, PID2019-111493RB-I00, PGC2018-096049-B-I00, CTQ2015-66194-R, PID2020-118893GB-I00 | es |
dc.description.sponsorship | Generalitat de Catalunya 2017-SGR-1079, 2017-SGR-1442, 2017-SGR-1189 | es |
dc.description.sponsorship | Junta de Andalucía US-1254317, US-1257019, P18-FR-3487, P18-HO-4091 | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | Springer Nature | es |
dc.relation.ispartof | Nature Communications, 13 (1), 7100. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Phosphorylation disrupts long-distance electron transport in cytochrome c | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | 945539 | es |
dc.relation.projectID | ICT-36-2020-101016787 | es |
dc.relation.projectID | PID2021-126663NB-I00 | es |
dc.relation.projectID | PID2019-111493RB-I00 | es |
dc.relation.projectID | PGC2018-096049-B-I00 | es |
dc.relation.projectID | CTQ2015-66194-R | es |
dc.relation.projectID | PID2020-118893GB-I00 | es |
dc.relation.projectID | 2017-SGR-1079 | es |
dc.relation.projectID | 2017-SGR-1442 | es |
dc.relation.projectID | 2017-SGR-1189 | es |
dc.relation.projectID | US-1254317 | es |
dc.relation.projectID | US-1257019 | es |
dc.relation.projectID | P18-FR-3487 | es |
dc.relation.projectID | P18-HO-4091 | es |
dc.relation.publisherversion | https://doi.org/10.1038/s41467-022-34809-1 | es |
dc.identifier.doi | 10.1038/s41467-022-34809-1 | es |
dc.journaltitle | Nature Communications | es |
dc.publication.volumen | 13 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 7100 | es |
dc.contributor.funder | European Union (UE) | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | Generalitat de Catalunya | es |
dc.contributor.funder | Junta de Andalucía | es |