Artículo
Natural Extracts Abolished Lipid Accumulation in Cells Harbouring non-favourable PNPLA3 genotype
Autor/es | Rojas, Ángela
Gallego, Paloma Gil Gómez, Antonio Muñoz Hernández, Rocío ![]() ![]() ![]() ![]() ![]() ![]() Martín, Lourdes Gallego Durán, Rocío García Valdecasas, Marta Campo, José A. del Bautista Palomas, Juan Dionisio ![]() ![]() ![]() ![]() ![]() ![]() Romero Gómez, Manuel ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Departamento | Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular |
Fecha de publicación | 2018 |
Fecha de depósito | 2022-07-05 |
Publicado en |
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Resumen | Background & aims. G-allele of PNPLA3 (rs738409) favours triglycerides accumulation and steatosis. In this study, we examined the effect of quercetin and natural extracts from mushroom and artichoke on reducing lipid ... Background & aims. G-allele of PNPLA3 (rs738409) favours triglycerides accumulation and steatosis. In this study, we examined the effect of quercetin and natural extracts from mushroom and artichoke on reducing lipid accumulation in hepatic cells. Material and methods. Huh7.5 cells were exposed to oleic acid (OA) and treated with quercetin and extracts to observe the lipid accumulation, the intracellular-TG concentration and the LD size. Sterol regulatory element binding proteins-1 (SREBP-1), peroxisome proliferator-activated receptor (PPARα-γ) and cholesterol acyltransferase (ACAT) gene expression levels were analysed. Results. Quercetin decreased the intracellular lipids, LD size and the levels of intracellular-TG through the down-regulation of SREBP-1c, PPARγand ACAT1 increasing PPARα. The natural-extracts suppressed OA-induced lipid accumulation and the intracellular-TG. They down-regulate the hepatic lipogenesis through SREBP-1c, besides the activation of lipolysis through the increasing of PPARα expression. Conclusions. Quercetin and the aqueous extracts decrease intracellular lipid accumulation by down-regulation of lipogenesis and up-regulation of lipolysis. |
Cita | Rojas, Á., Gallego, P., Gil Gómez, A., Muñoz Hernández, R., Martín, L., Gallego Durán, R.,...,Romero Gómez, M. (2018). Natural Extracts Abolished Lipid Accumulation in Cells Harbouring non-favourable PNPLA3 genotype. Annals of Hepatology, 17 (2), 242-249. |
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