dc.creator | Medrano Padial, Concepción | es |
dc.creator | Puerto Rodríguez, María | es |
dc.creator | Merchán Gragero, María del Mar | es |
dc.creator | Moreno Onorato, Francisco Javier | es |
dc.creator | Richard, Tristan | es |
dc.creator | Cantos Villar, Emma | es |
dc.creator | Pichardo Sánchez, Silvia | es |
dc.date.accessioned | 2021-12-21T12:52:37Z | |
dc.date.available | 2021-12-21T12:52:37Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Medrano Padial, C., Puerto Rodríguez, M., Merchán Gragero, M.d.M., Moreno Onorato, F.J., Richard, T., Cantos Villar, E. y Pichardo Sánchez, S. (2020). Cytotoxicity studies of a stilbene extract and its main components intended to be used as preservative in the wine industry. Food Research International, 137, 109738. | |
dc.identifier.issn | 0963-9969 | es |
dc.identifier.uri | https://hdl.handle.net/11441/128532 | |
dc.description.abstract | The use of stilbenes has been proposed as an alternative to sulfur dioxide in wine. Provided the feasibility from a technological approach, the cytotoxicity of an extract from grapevine shoots containing a stilbene richness of 99% (ST-99 extract) was assessed in the human cell lines HepG2 and Caco-2. In addition, the effects of the main stilbenes found in ST-99, trans-resveratrol and trans-ε-viniferin were studied, as well as its mixture. Similar cytotoxic effects were obtained in the exposures to trans-ε-viniferin, ST-99 and the mixture; however, trans-resveratrol alone exerted less toxicity. When HepG2 cells were exposed to trans-ε-viniferin, ST-99 and the mixture, the mean effective concentration (EC50) were 28.28 ± 2.15, 31.91 ± 1.55 and 29.47 ± 3.54 µg/mL, respectively. However, in the exposure to trans-resveratrol, the EC50 was higher 50 µg/mL. The morphological study evidenced damage at ultrastructural level in HepG2 cells, highlighting the inhibition of cell proliferation and the induction of apoptosis. The type of interaction produced by trans-ε-viniferin and trans-resveratrol mixtures was assessed by an isobologram analysis using the CalcuSyn software, evidencing an antagonist effect. These data comprise a starting point in the toxicological assessment; further studies are needed in this field to assure the safety of the extract ST-99. | es |
dc.description.sponsorship | Ministerio de Economía, Industria y Competitividad and INIA (RTA2015-00005-C02-02) | es |
dc.description.sponsorship | Bordeaux Metabolome Facility and MetaboHUB (ANR-11-INBS-0010 project) | es |
dc.format | application/pdf | es |
dc.format.extent | 10 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Food Research International, 137, 109738. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Toxicity | es |
dc.subject | Stilbene | es |
dc.subject | Wine | es |
dc.subject | Trans-resveratrol | es |
dc.subject | Trans-ε-viniferin | es |
dc.title | Cytotoxicity studies of a stilbene extract and its main components intended to be used as preservative in the wine industry | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.relation.projectID | RTA2015-00005-C02-02 | es |
dc.relation.projectID | ANR-11-INBS-0010 | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.foodres.2020.109738 | es |
dc.identifier.doi | 10.1016/j.foodres.2020.109738 | es |
dc.journaltitle | Food Research International | es |
dc.publication.volumen | 137 | es |
dc.publication.initialPage | 109738 | es |
dc.contributor.funder | Ministerio de Economia, Industria y Competitividad (MINECO). España | es |
dc.contributor.funder | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) | es |