Article
Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast
Author/s | Jaafar, Mariam
Contreras Fernández, Julia Mª Dominique, Carine Martín Villanueva, Sara ![]() ![]() ![]() Capeyrou, Régine Vitali, Patrice Rodríguez Galán, Olga Velasco Ramírez, María del Carmen ![]() ![]() ![]() ![]() ![]() ![]() Humbert, Odile Watkins, Nicholas J. Villalobo Polo, Eduardo ![]() ![]() ![]() ![]() ![]() ![]() ![]() Bohnsack, Katherine E. Cruz Díaz, Jesús de la ![]() ![]() ![]() ![]() ![]() ![]() ![]() Henras, Anthony K. |
Department | Universidad de Sevilla. Departamento de Genética Universidad de Sevilla. Departamento de Microbiología |
Publication Date | 2021 |
Deposit Date | 2021-11-08 |
Published in |
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Abstract | Synthesis of eukaryotic ribosomes involves the assembly and maturation of precursor particles (pre-ribosomal particles) containing ribosomal RNA (rRNA) precursors, ribosomal proteins (RPs) and a plethora of assembly factors ... Synthesis of eukaryotic ribosomes involves the assembly and maturation of precursor particles (pre-ribosomal particles) containing ribosomal RNA (rRNA) precursors, ribosomal proteins (RPs) and a plethora of assembly factors (AFs). Formation of the earliest precursors of the 60S ribosomal subunit (pre-60S r-particle) is among the least understood stages of ribosome biogenesis. It involves the Npa1 complex, a protein module suggested to play a key role in the early structuring of the pre-rRNA. Npa1 displays genetic interactions with the DExD-box protein Dbp7 and interacts physically with the snR190 box C/D snoRNA. We show here that snR190 functions as a snoRNA chaperone, which likely cooperates with the Npa1 complex to initiate compaction of the pre-rRNA in early pre-60S r-particles. We further show that Dbp7 regulates the dynamic base-pairing between snR190 and the pre-rRNA within the earliest pre-60S r-particles, thereby participating in structuring the peptidyl transferase center (PTC) of the large ribosomal subunit. |
Project ID. | PID2019-103859-GB-I00
![]() AEI/ 10.13039/501100011033 ![]() SFB860 ![]() |
Citation | Jaafar, M., Contreras Fernández, J.M., Dominique, C., Martín Villanueva, S., Capeyrou, R., Vitali, P.,...,Henras, A.K. (2021). Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast. Nature Communications, 12 (1), 6153. |
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