Artículo
Use of Impedance Spectroscopy for the Characterization of In-Vitro Osteoblast Cell Response in Porous Titanium Bone Implants
Autor/es | Giner García, Mercedes
Olmo Fernández, Alberto Hernández, Miguel Trueba Muñoz, Paloma Chicardi Augusto, Ernesto Civantos, Ana Vázquez Gámez, María de los Ángeles Montoya García, María José Torres Hernández, Yadir |
Departamento | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica Universidad de Sevilla. Departamento de Tecnología Electrónica Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte Universidad de Sevilla. Departamento de Medicina |
Fecha de publicación | 2020-08 |
Fecha de depósito | 2020-09-09 |
Publicado en |
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Premios | Premio Mensual Publicación Científica Destacada de la US. Escuela Politécnica Superior |
Resumen | The use of titanium implants with adequate porosity (content, size and morphology) could solve the stress shielding limitations that occur in conventional titanium implants. Experiments to assess the cellular response ... The use of titanium implants with adequate porosity (content, size and morphology) could solve the stress shielding limitations that occur in conventional titanium implants. Experiments to assess the cellular response (adhesion, proliferation and differentiation of osteoblasts) on implants are expensive, time-consuming and delicate. In this work, we propose the use of impedance spectroscopy to evaluate the growth of osteoblasts on porous titanium implants. Osteoblasts cells were cultured on fully-dense and 40 vol.% porous discs with two ranges of pore size (100–200 μm and 355–500 μm) to study cell viability, proliferation, differentiation (Alkaline phosphatase activity) and cell morphology. The porous substrates 40 vol.% (100–200 µm) showed improved osseointegration response as achieved more than 80% of cell viability and higher levels of Cell Differentiation by Alkaline Phosphatase (ALP) at 21 days. This cell behavior was further evaluated observing an increase in the impedance modulus for all study conditions when cells were attached. However, impedance levels were higher on fully-dense due to its surface properties (flat surface) than porous substrates (flat and pore walls). Surface parameters play an important role on the global measured impedance. Impedance is useful for characterizing cell cultures in different sample types. |
Identificador del proyecto | PID2019-109371GB-I00
US-1259771 P18-FR-2038 |
Cita | Giner García, M., Olmo Fernández, A., Hernández, M., Trueba Muñoz, P., Chicardi Augusto, E., Civantos, A.,...,Torres Hernández, Y. (2020). Use of Impedance Spectroscopy for the Characterization of In-Vitro Osteoblast Cell Response in Porous Titanium Bone Implants. Metals, 10 (8), 1077-. |
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M_trueba-muñoz_2020_Use-of.pdf | 3.626Mb | [PDF] | Ver/ | |