Artículo
Phosphorylation disrupts long-distance electron transport in cytochrome c
Autor/es | Gomila, Alexandre M.J.
Pérez Mejías, Gonzalo Nin-Hill, Alba Guerra Castellano, Alejandra ![]() ![]() ![]() ![]() ![]() Casas Ferrer, Laura Ortiz Tescari, Sthefany Díaz Quintana, Antonio Jesús ![]() ![]() ![]() ![]() ![]() ![]() ![]() Samitier, Josep Rovira, Carme Rosa Acosta, Miguel Ángel de la ![]() ![]() ![]() ![]() ![]() ![]() Díaz Moreno, Irene ![]() ![]() ![]() ![]() ![]() ![]() ![]() Gorostiza, Pau Giannotti, Marina I. Lagunas, Anna |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2022 |
Fecha de depósito | 2022-12-07 |
Publicado en |
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Resumen | t has been recently shown that electron transfer between mitochondrial cytochrome c and the cytochrome c1 subunit of the cytochrome bc1 can proceed at long-distance through the aqueous solution. Cytochrome c is thought to ... t has been recently shown that electron transfer between mitochondrial cytochrome c and the cytochrome c1 subunit of the cytochrome bc1 can proceed at long-distance through the aqueous solution. Cytochrome c is thought to adjust its activity by changing the affinity for its partners via Tyr48 phosphorylation, but it is unknown how it impacts the nanoscopic environment, interaction forces, and long-range electron transfer. Here, we constrain the orientation and separation between cytochrome c1 and cytochrome c or the phosphomimetic Y48pCMF cytochrome c, and deploy an array of single-molecule, bulk, and computational methods to investigate the molecular mechanism of electron transfer regulation by cytochrome c phosphorylation. We demonstrate that phosphorylation impairs long-range electron transfer, shortens the long-distance charge conduit between the partners, strengthens their interaction, and departs it from equilibrium. These results unveil a nanoscopic view of the interaction between redox protein partners in electron transport chains and its mechanisms of regulation. |
Agencias financiadoras | European Union (UE) Ministerio de Ciencia e Innovación (MICIN). España Generalitat de Catalunya Junta de Andalucía |
Identificador del proyecto | 945539
![]() ICT-36-2020-101016787 ![]() PID2021-126663NB-I00 ![]() PID2019-111493RB-I00 ![]() PGC2018-096049-B-I00 ![]() CTQ2015-66194-R ![]() PID2020-118893GB-I00 ![]() 2017-SGR-1079 ![]() 2017-SGR-1442 ![]() 2017-SGR-1189 ![]() US-1254317 ![]() US-1257019 ![]() P18-FR-3487 ![]() P18-HO-4091 ![]() |
Cita | Gomila, A.M.J., Pérez Mejías, G., Nin-Hill, A., Guerra Castellano, A., Casas Ferrer, L., Ortiz Tescari, S.,...,Lagunas, A. (2022). Phosphorylation disrupts long-distance electron transport in cytochrome c. Nature Communications, 13 (1), 7100. https://doi.org/10.1038/s41467-022-34809-1. |
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Phosphorylation disrupts.pdf | 3.338Mb | ![]() | Ver/ | |