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The Helicase PIF1 Facilitates Resection over Sequences Prone to Forming G4 Structures

 

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Opened Access The Helicase PIF1 Facilitates Resection over Sequences Prone to Forming G4 Structures
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Author: Mejías Navarro, Fernando
Jimeno González, Sonia
Fernández Ávila, Mª Jesús
Soria Bretones, Isabel
Prados Carvajal, Rosario
Camarillo, Rosa
Department: Universidad de Sevilla. Departamento de Genética
Date: 2018
Document type: Article
Abstract: DNA breaks are complex lesions that can be repaired either by non-homologous end joining (NHEJ) or by homologous recombination (HR). The decision between these two routes of DNA repair is a key point of the DNA damage response (DDR) that is controlled by DNA resection. The core machinery catalyzing the resection process is well established. However, little is known about the additional requirements of DNA resection over DNA structures with high complexity. Here, we found evidence that the human helicase PIF1 has a role in DNA resection, specifically for defined DNA regions, such as those prone to form G-quadruplexes. Indeed, PIF1 is recruited to the site of DNA damage and physically interacts with proteins involved in DNA resection, and its depletion causes DNA damage sensitivity and a reduction of HR efficiency. Moreover, G4 stabilization by itself hampers DNA resection, a phenomenon suppressed by PIF1 overexpression
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URI: https://hdl.handle.net/11441/85720

DOI: 10.1016/j.celrep.2018.08.047

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