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Modulation of copper site properties by remote residues determines the stability of plastocyanins

 

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Opened Access Modulation of copper site properties by remote residues determines the stability of plastocyanins
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Author: Muñoz López, Francisco Jesús
Frutos Beltrán, Estrella
Díaz Moreno, Sofía
Díaz Moreno, Irene
Subías Peruga, Maria Gloria
Rosa Acosta, Miguel Ángel de la
Díaz Quintana, Antonio Jesús
Department: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Date: 2010
Published in: FEBS Letters, 584 (11), 2346-2350.
Document type: Article
Abstract: The metal cofactor determines the thermal stability in cupredoxins, but how the redox state of copper modulates their melting points remains unknown. The metal coordination environment is highly conserved in cyanobacterial plastocyanins. However, the oxidised form is more stable than the reduced one in thermophilic Phormidium, but the opposite occurs in mesophilic Synechocystis. We have performed neutral amino‐acid substitutions at loops of Phormidium plastocyanin far from the copper site. Notably, mutation P49G/G50P confers a redox‐dependent thermal stability similar to that of the mesophilic plastocyanin. Moreover, X‐ray absorption spectroscopy reveals that P49G/G50P mutation makes the electron density distribution at the oxidised copper site shift towards that of Synechocystis plastocyanin.
Cite: Muñoz López, F.J., Frutos Beltrán, E., Díaz Moreno, S., Díaz Moreno, I., Subías Peruga, M.G., Rosa Acosta, M.Á.d.l. y Díaz Quintana, A.J. (2010). Modulation of copper site properties by remote residues determines the stability of plastocyanins. FEBS Letters, 584 (11), 2346-2350.
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URI: https://hdl.handle.net/11441/84780

DOI: 10.1016/j.febslet.2010.04.013

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