dc.creator | Bermejo, Rodrigo | es |
dc.creator | Gómez González, Belén | es |
dc.creator | Aguilera López, Andrés | es |
dc.creator | Foiani, Marco | es |
dc.creator | Capra, Thelma | es |
dc.creator | Cocito, Andrea | es |
dc.date.accessioned | 2018-03-19T18:20:53Z | |
dc.date.available | 2018-03-19T18:20:53Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Bermejo, R., Gómez González, B., Aguilera López, A., Foiani, M., Capra, T. y Cocito, A. (2011). The replication checkpoint protects fork stability by releasing transcribed genes from nuclear pores. Cell, 146 (2), 233-246. | |
dc.identifier.issn | 0092-8674 (impreso) | es |
dc.identifier.issn | 1097-4172 (electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/71106 | |
dc.description.abstract | Transcription hinders replication fork progression and stability, and the Mec1/ATR checkpoint protects fork integrity. Examining checkpoint-dependent mechanisms controlling fork stability, we find that fork reversal and dormant origin firing due to checkpoint defects are rescued in checkpoint mutants lacking THO, TREX-2, or inner-basket nucleoporins. Gene gating tethers transcribed genes to the nuclear periphery and is counteracted by checkpoint kinases through phosphorylation of nucleoporins such as Mlp1. Checkpoint mutants fail to detach transcribed genes from nuclear pores, thus generating topological impediments for incoming forks. Releasing this topological complexity by introducing a double-strand break between a fork and a transcribed unit prevents fork collapse. Mlp1 mutants mimicking constitutive checkpoint-dependent phosphorylation also alleviate checkpoint defects. We propose that the checkpoint assists fork progression and stability at transcribed genes by phosphorylating key nucleoporins and counteracting gene gating, thus neutralizing the topological tension generated at nuclear pore gated genes. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación BFU2006-05260, 2010 CSD2007-0015 | es |
dc.description.sponsorship | National Institutes of Health GM053738 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Cell, 146 (2), 233-246. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cell cycle proteins | es |
dc.subject | Hydroxyurea | es |
dc.subject | RAD53 protein | es |
dc.subject | Threonine kinases | es |
dc.title | The replication checkpoint protects fork stability by releasing transcribed genes from nuclear pores | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BFU2006-05260 | es |
dc.relation.projectID | 2010 CSD2007-0015 | es |
dc.relation.projectID | GM053738 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.cell.2011.06.033 | es |
dc.identifier.doi | 10.1016/j.cell.2011.06.033 | es |
idus.format.extent | 14 p. | es |
dc.journaltitle | Cell | es |
dc.publication.volumen | 146 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 233 | es |
dc.publication.endPage | 246 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | |
dc.contributor.funder | National Institutes of Health. United States | |