dc.creator | Gómez Herreros, Fernando | es |
dc.creator | Zagnoli Vieira, Guido | es |
dc.creator | Ntai, Ioanna | es |
dc.creator | Martínez Macías, María Isabel | es |
dc.creator | Anderson, Rhona M. | es |
dc.creator | Herrero Ruíz, Andrés | es |
dc.creator | Caldecott, Keith W. | es |
dc.date.accessioned | 2017-08-21T10:16:00Z | |
dc.date.available | 2017-08-21T10:16:00Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Gómez Herreros, F., Zagnoli Vieira, G., Ntai, I., Martínez Macías, M.I., Anderson, R.M., Herrero Ruíz, A. y Caldecott, .W. (2017). TDP2 suppresses chromosomal translocations induced by DNA topoisomerase II during gene transcription. Nature Communications, 8 (233), 1-8. | |
dc.identifier.issn | 2041-1723 | es |
dc.identifier.uri | http://hdl.handle.net/11441/63912 | |
dc.description.abstract | DNA double-strand breaks (DSBs) induced by abortive topoisomerase II (TOP2) activity are a potential source of genome instability and chromosome translocation. TOP2-induced DNA double-strand breaks are rejoined in part by tyrosyl-DNA phosphodiesterase 2 (TDP2)-dependent non-homologous end-joining (NHEJ), but whether this process suppresses or promotes TOP2-induced translocations is unclear. Here, we show that TDP2 rejoins DSBs induced during transcription-dependent TOP2 activity in breast cancer cells and at the translocation ‘hotspot’, MLL. Moreover, we find that TDP2 suppresses chromosome rearrangements induced by TOP2 and reduces TOP2-induced chromosome translocations that arise during gene transcription. Interestingly, however, we implicate TDP2-dependent NHEJ in the formation of a rare subclass of translocations associated previously with therapy-related leukemia and characterized by junction sequences with 4-bp of perfect homology. Collectively, these data highlight the threat posed by TOP2-induced DSBs during transcription and demonstrate the importance of TDP2-dependent non-homologous end-joining in protecting both gene transcription and genome stability. | es |
dc.description.sponsorship | España, MINECO RYC-2014-16665 | es |
dc.description.sponsorship | España, MINECO BFU2016-76446-P | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Nature Publishing Group | es |
dc.relation.ispartof | Nature Communications, 8 (233), 1-8. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | TDP2 suppresses chromosomal translocations induced by DNA topoisomerase II during gene transcription | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/RYC-2014-16665 | es |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/BFU2016-76446-P | es |
dc.relation.publisherversion | http://dx.doi.org/10.1038/s41467-017-00307-y | es |
dc.identifier.doi | 10.1038/s41467-017-00307-y | es |
idus.format.extent | 9 p. | es |
dc.journaltitle | Nature Communications | es |
dc.publication.volumen | 8 | es |
dc.publication.issue | 233 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 8 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | |