Artículo
R-Loop Mediated Transcription-Associated
Recombination in trf4D Mutants Reveals New Links
between RNA Surveillance and Genome Integrity
Autor/es | Gavaldá Martín, Sandra
Gallardo, Mercedes Luna Varo, Rosa María Aguilera López, Andrés |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2013 |
Fecha de depósito | 2017-07-10 |
Publicado en |
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Resumen | To get further insight into the factors involved in the maintenance of genome integrity we performed a screening of
Saccharomyces cerevisiae
deletion strains inducing hyperrecombination. We have identified
trf4
, a ... To get further insight into the factors involved in the maintenance of genome integrity we performed a screening of Saccharomyces cerevisiae deletion strains inducing hyperrecombination. We have identified trf4 , a gene encoding a non- canonical polyA-polymerase involved in RNA surveillance, as a factor that prevents recombination between DNA repeats. We show that trf4 D confers a transcription-associated recombination phenotype that is mediated by the nascent mRNA. In addition, trf4 D also leads to an increase in the mutation frequency. Both genetic instability phenotypes can be suppressed by overexpression of RNase H and are exacerbated by overexpression of the human cytidine deaminase AID. These results suggest that in the absence of Trf4 R-loops accumulate co-transcriptionally increasing the recombination and mutation frequencies. Altogether our data indicate that Trf4 is necessary for both mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism in S. cerevisiae |
Cita | Gavaldá Martín, S., Gallardo, M., Luna Varo, R.M. y Aguilera López, A. (2013). R-Loop Mediated Transcription-Associated Recombination in trf4D Mutants Reveals New Links between RNA Surveillance and Genome Integrity. PLoS ONE, 8 (6), 65541. |
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