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A genome-wide function of THSC/TREX-2 at active genes prevents transcription–replication collisions

Opened Access A genome-wide function of THSC/TREX-2 at active genes prevents transcription–replication collisions

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Autor: Santos Pereira, José María
García Rubio, María Luisa
González Aguilera, Cristina
Luna Varo, Rosa María
Aguilera López, Andrés
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2014
Publicado en: Nucleic acids research, 42(19), 12000-12014
Tipo de documento: Artículo
Resumen: The THSC/TREX-2 complex of Saccharomyces cerevisiae mediates the anchoring of transcribed genes to the nuclear pore, linking transcription elongation with mRNA export and genome stability, as shown for specific reporters. However, it is still unknown whether the function of TREX-2 is global and the reason for its relevant role in genome integrity. Here, by studying two TREX-2 representative subunits, Thp1 and Sac3, we show that TREX-2 has a genome-wide role in gene expression. Both proteins show similar distributions along the genome, with a gradient disposition at active genes that increases towards the 3′ end. Thp1 and Sac3 have a relevant impact on the expression of long, G+C-rich and highly transcribed genes. Interestingly, replication impairment detected by the genome-wide accumulation of the replicative Rrm3 helicase is increased preferentially at highly expressed genes in the thp1Δ and sac3Δ mutants analyzed. Therefore, our work provides evidence of a function of TREX-2 at the ...
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Tamaño: 5.643Mb
Formato: PDF

URI: http://hdl.handle.net/11441/29095

DOI: http://dx.doi.org/10.1093/nar/gku906

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