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Secretory responses of intact glomus cells in thin slices of rat carotid body to hypoxia and tetraethylammonium

Opened Access Secretory responses of intact glomus cells in thin slices of rat carotid body to hypoxia and tetraethylammonium

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Autor: Pardal Redondo, Ricardo
Ludewig, Uwe
García Hirschfeld, Julia
López Barneo, José
Departamento: Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica
Fecha: 2000
Publicado en: The proceedings of the National Academy of Sciences of the United States of America, 97 (51), 2361-2366.
Tipo de documento: Artículo
Resumen: We have developed a thin-slice preparation of whole rat carotid body that allows us to perform patch-clamp recording of membrane ionic currents and to monitor catecholamine secretion by amperometry in single glomus cells under direct visual control. In normoxic conditions (PO2 ' 140 mmHg; 1 mmHg 5 133 Pa), most glomus cells did not have measurable secretory activity, but exposure to hypoxia (PO2 ' 20 mmHg) elicited the appearance of a large number of spike-like exocytotic events. This neurosecretory response to hypoxia was fully reversible and required extracellular Ca21 influx. The average charge of single quantal events was 46 6 25 fC (n 5 218), which yields an estimate of '140,000 catecholamine molecules per vesicle. Addition of tetraethylammonium (TEA; 2–5 mM) to the extracellular solution induced in most (>95%) cells tested (n 5 32) a secretory response similar to that elicited by low PO2. Cells nonresponsive to hypoxia but activated by exposure to high external K1 were also stim...
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Tamaño: 275.2Kb
Formato: PDF

URI: http://hdl.handle.net/11441/17785

DOI: 10.1073/pnas.030522297

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