dc.creator | Zatreanu, Diana | es |
dc.creator | Han, Zhong | es |
dc.creator | Mitter, Richard | es |
dc.creator | Tumini, Emanuela | es |
dc.creator | Williams, Hannah C. | es |
dc.creator | Gregersen, Lea Haarup | es |
dc.creator | Dirac-Svejstrup, A. Barbara | es |
dc.creator | Roma, Stefania | es |
dc.creator | Stewart, Aengus | es |
dc.creator | Aguilera López, Andrés | es |
dc.creator | Svejstrup, Jesper | es |
dc.date.accessioned | 2019-12-04T15:07:30Z | |
dc.date.available | 2019-12-04T15:07:30Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Zatreanu, D., Han, Z., Mitter, R., Tumini, E., Williams, H.C., Gregersen, L.H.,...,Svejstrup, J. (2019). Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability. Molecular Cell, 76 (1), 57-69.e9. | |
dc.identifier.issn | 1097-2765 | es |
dc.identifier.issn | 1097-4164 | es |
dc.identifier.uri | https://hdl.handle.net/11441/90721 | |
dc.description.abstract | Although correlations between RNA polymerase II (RNAPII) transcription stress, R-loops, and genome instability have been established, the mechanisms underlying these connections remain poorly understood. Here, we used a mutant version of the transcription elongation factor TFIIS (TFIISmut), aiming to specifically induce increased levels of RNAPII pausing, arrest, and/or backtracking in human cells. Indeed, TFIISmut expression results in slower elongation rates, relative depletion of polymerases from the end of genes, and increased levels of stopped RNAPII; it affects mRNA splicing and termination as well. Remarkably, TFIISmut expression also dramatically increases R-loops, which may form at the anterior end of backtracked RNAPII and trigger genome instability, including DNA strand breaks. These results shed light on the relationship between transcription stress and R-loops and suggest that different classes of R-loops may exist, potentially with distinct consequences for genome stability. | es |
dc.description.sponsorship | Cancer Research UK FC001166 | es |
dc.description.sponsorship | UK Medical Research Council FC001166 | es |
dc.description.sponsorship | Wellcome Trust FC001166 | es |
dc.description.sponsorship | European Research Council 693327, ERC2014 AdG669898 | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BFU2013-42918-P, BFU2016-75058-P | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Molecular Cell, 76 (1), 57-69.e9. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | 53BP1 | es |
dc.subject | Backtracking | es |
dc.subject | DNA-RNA hybrids | es |
dc.subject | R-loops | es |
dc.subject | RNA polymerase II | es |
dc.subject | TFIIS | es |
dc.subject | Transcript cleavage | es |
dc.subject | Transcript elongation | es |
dc.subject | Transcription-associated genome instability | es |
dc.title | Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | FC001166 | es |
dc.relation.projectID | FC001166 | es |
dc.relation.projectID | FC001166 | es |
dc.relation.projectID | 693327 | es |
dc.relation.projectID | ERC2014 AdG669898 | es |
dc.relation.projectID | BFU2013-42918-P | es |
dc.relation.projectID | BFU2016-75058-P | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.molcel.2019.07.037 | es |
dc.identifier.doi | 10.1016/j.molcel.2019.07.037 | es |
idus.format.extent | 22 p. | es |
dc.journaltitle | Molecular Cell | es |
dc.publication.volumen | 76 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 57 | es |
dc.publication.endPage | 69.e9 | es |