Article
A portable epigenetic switch for bistable gene expression in bacteria
Author/s | Rodríguez Olivenza, David
Sánchez Romero, María Antonia Cota García, Ignacio Casadesús Pursals, Josep Nicoloff, Hervé Andersson, Dan I. |
Department | Universidad de Sevilla. Departamento de Genética |
Publication Date | 2019 |
Deposit Date | 2019-09-09 |
Published in |
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Abstract | We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream ... We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered opvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics. |
Project ID. | grant BIO2016-75235-P |
Citation | Rodríguez Olivenza, D., Sánchez Romero, M.A., Cota García, I., Casadesús Pursals, J., Nicoloff, H. y Andersson, D.I. (2019). A portable epigenetic switch for bistable gene expression in bacteria. Scientific Reports, 9 (1), 11261. |
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