A portable epigenetic switch for bistable gene expression in bacteria

Rodríguez Olivenza, David; Sánchez Romero, María Antonia; Cota García, Ignacio; Casadesús Pursals, Josep; Nicoloff, Hervé; Andersson, Dan I.

doi:10.1038/s41598-019-47650-2

Artículo

dc.creator	Rodríguez Olivenza, David	es
dc.creator	Sánchez Romero, María Antonia	es
dc.creator	Cota García, Ignacio	es
dc.creator	Casadesús Pursals, Josep	es
dc.creator	Nicoloff, Hervé	es
dc.creator	Andersson, Dan I.	es
dc.date.accessioned	2019-09-09T11:38:57Z
dc.date.available	2019-09-09T11:38:57Z
dc.date.issued	2019
dc.identifier.citation	Rodríguez Olivenza, D., Sánchez Romero, M.A., Cota García, I., Casadesús Pursals, J., Nicoloff, H. y Andersson, D.I. (2019). A portable epigenetic switch for bistable gene expression in bacteria. Scientific Reports, 9 (1), 11261.
dc.identifier.issn	2045-2322	es
dc.identifier.uri	https://hdl.handle.net/11441/89064
dc.description.abstract	We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered opvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics.	es
dc.description.sponsorship	Ministerio de Ciencia, Innovación y Universidades of grant BIO2016-75235-P	es
dc.format	application/pdf	es
dc.language.iso	eng	es
dc.publisher	Nature Research	es
dc.relation.ispartof	Scientific Reports, 9 (1), 11261.
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Bacterial synthetic biology	es
dc.subject	DNA methylation	es
dc.title	A portable epigenetic switch for bistable gene expression in bacteria	es
dc.type	info:eu-repo/semantics/article	es
dcterms.identifier	https://ror.org/03yxnpp24
dc.type.version	info:eu-repo/semantics/publishedVersion	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	es
dc.contributor.affiliation	Universidad de Sevilla. Departamento de Genética	es
dc.relation.projectID	grant BIO2016-75235-P	es
dc.relation.publisherversion	http://dx.doi.org/10.1038/s41598-019-47650-2	es
dc.identifier.doi	10.1038/s41598-019-47650-2	es
idus.format.extent	9 p.	es
dc.journaltitle	Scientific Reports	es
dc.publication.volumen	9	es
dc.publication.issue	1	es
dc.publication.initialPage	11261	es

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