dc.creator | Salas Armenteros, Irene | es |
dc.creator | Barroso Ceballos, Sonia Inés | es |
dc.creator | García Rondón, Ana | es |
dc.creator | Pérez Calero, Mónica | es |
dc.creator | Andújar, Eloísa | es |
dc.creator | Luna Varo, Rosa María | es |
dc.creator | Aguilera López, Andrés | |
dc.date.accessioned | 2019-09-09T11:24:11Z | |
dc.date.available | 2019-09-09T11:24:11Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Salas Armenteros, I., Barroso Ceballos, S.I., García Rondón, A., Pérez Calero, M., Andújar, E., Luna Varo, R.M. y Aguilera López, A. (2019). Depletion of the MFAP1/SPP381 Splicing Factor Causes R-Loop-Independent Genome Instability. Cell Reports, 28 (6), 1551-1563. | |
dc.identifier.issn | 2211-1247 | es |
dc.identifier.uri | https://hdl.handle.net/11441/89062 | |
dc.description.abstract | THO/TREX is a conserved complex with a role in messenger ribonucleoprotein biogenesis that links gene expression and genome instability. Here, we show that human THO interacts with MFAP1 (microfibrillar-associated protein 1), a spliceosome-associated factor. Interestingly, MFAP1 depletion impairs cell proliferation and genome integrity, increasing γH2AX foci and DNA breaks. This phenotype is not dependent on either transcription or RNA-DNA hybrids. Mutations in the yeast orthologous gene SPP381 cause similar transcription-independent genome instability, supporting a conserved role. MFAP1 depletion has a wide effect on splicing and gene expression in human cells, determined by transcriptome analyses. MFAP1 depletion affects a number of DNA damage response (DDR) genes, which supports an indirect role of MFAP1 on genome integrity. Our work defines a functional interaction between THO and RNA processing and argues that splicing factors may contribute to genome integrity indirectly by regulating the expression of DDR genes rather than by a direct role. | es |
dc.description.sponsorship | European ResearchCouncil (grant ERC2014 AdG669898 TARLOOP) | es |
dc.description.sponsorship | Junta de Andalucía Spain (grant BIO1238) | es |
dc.description.sponsorship | Spanish Ministry of Economy and Competitiveness (grant BFU2016-75058-P) | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Cell Reports, 28 (6), 1551-1563. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | THO complex | es |
dc.subject | alernative splicing | es |
dc.subject | MFAP1/SPP381 | es |
dc.subject | genome instability | es |
dc.title | Depletion of the MFAP1/SPP381 Splicing Factor Causes R-Loop-Independent Genome Instability | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | ERC2014 AdG669898 TARLOOP | es |
dc.relation.projectID | grant BFU2016-75058-P | es |
dc.relation.projectID | grant BIO1238 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.celrep.2019.07.010 | es |
dc.identifier.doi | 10.1016/j.celrep.2019.07.010 | es |
idus.format.extent | 12 p. | es |
dc.journaltitle | Cell Reports | es |
dc.publication.volumen | 28 | es |
dc.publication.issue | 6 | es |
dc.publication.initialPage | 1551 | es |
dc.publication.endPage | 1563 | es |