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dc.creatorRodríguez Martínez, Danieles
dc.creatorMartínez Losa, Magdalenaes
dc.creatorÁlvarez Dolado, Manueles
dc.date.accessioned2017-09-08T14:57:58Z
dc.date.available2017-09-08T14:57:58Z
dc.date.issued2017
dc.identifier.citationRodríguez Martínez, D., Martínez Losa, M. y Álvarez Dolado, M. (2017). Cryopreservation of GABAergic neuronal precursors for cell-based therapy. Plos One, 12 (1), e0170776-.
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/11441/64294
dc.description.abstractCryopreservation protocols are essential for stem cells storage in order to apply them in the clinic. Here we describe a new standardized cryopreservation protocol for GABAergic neural precursors derived from the medial glanglionic eminence (MGE), a promising source of GABAergic neuronal progenitors for cell therapy against interneuron-related pathologies. We used 10% Me2 SO as cryoprotectant and assessed the effects of cell culture amplification and cellular organization, as in toto explants, neurospheres, or individualized cells, on post-thaw cell viability and retrieval. We confirmed that in toto cryopreservation of MGE explants is an optimal preservation system to keep intact the interneuron precursor properties for cell transplantation, together with a high cell viability (>80%) and yield (>70%). Postthaw proliferation and self-renewal of the cryopreserved precursors were tested in vitro. In addition, their migration capacity, acquisition of mature neuronal morphology, and potency to differentiate into multiple interneuron subtypes were also confirmed in vivo after transplantation. The results show that the cryopreserved precursor features remained intact and were similar to those immediately transplanted after their dissection from the MGE. We hope this protocol will facilitate the generation of biobanks to obtain a permanent and reliable source of GABAergic precursors for clinical application in cell-based therapies against interneuronopathies.es
dc.description.sponsorshipMinisterio de Economía y Competitividad 09-07746 y 12-36853es
dc.description.sponsorshipJunta de Andalucía CTS- 2563es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPlos One, 12 (1), e0170776-.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleCryopreservation of GABAergic neuronal precursors for cell-based therapyes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/09-07746es
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/12-36853es
dc.relation.projectIDCTS- 2563es
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0170776es
dc.identifier.doi10.1371/journal.pone.0170776es
idus.format.extent17 p.es
dc.journaltitlePlos Onees
dc.publication.volumen12es
dc.publication.issue1es
dc.publication.initialPagee0170776es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). España
dc.contributor.funderJunta de Andalucía

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