Article
A genome-wide screening uncovers the role of CCAR2 as an antagonist of DNA end resection
Author/s | López Saavedra, Ana
Gómez Cabello, Daniel ![]() ![]() ![]() ![]() ![]() ![]() Domínguez Sánchez, María Salud Mejías Navarro, Fernando Fernández Ávila, María Jesús Martínez Macías, María Isabel Huertas Sánchez, Pablo ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Department | Universidad de Sevilla. Departamento de Genética |
Publication Date | 2016 |
Deposit Date | 2017-03-17 |
Published in |
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Abstract | There are two major and alternative pathways to repair DNA double-strand breaks: non-homologous end-joining and homologous recombination. Here we identify and characterize novel factors involved in choosing between these ... There are two major and alternative pathways to repair DNA double-strand breaks: non-homologous end-joining and homologous recombination. Here we identify and characterize novel factors involved in choosing between these pathways; in this study we took advantage of the SeeSaw Reporter, in which the repair of double-strand breaks by homology-independent or -dependent mechanisms is distinguished by the accumulation of green or red fluorescence, respectively. Using a genome-wide human esiRNA (endoribonuclease-prepared siRNA) library, we isolate genes that control the recombination/end-joining ratio. Here we report that two distinct sets of genes are involved in the control of the balance between NHEJ and HR: those that are required to facilitate recombination and those that favour NHEJ. This last category includes CCAR2/DBC1, which we show inhibits recombination by limiting the initiation and the extent of DNA end resection, thereby acting as an antagonist of CtIP. |
Citation | López Saavedra, A., Gómez Cabello, D., Domínguez Sánchez, M.S., Mejías Navarro, F., Fernández Ávila, M.J., Martínez Macías, M.I. y Huertas Sánchez, P. (2016). A genome-wide screening uncovers the role of CCAR2 as an antagonist of DNA end resection. Nature Communications, 7 (12364), 1-14. |
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