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Artículo
A positioned +1 nucleosome enhances promoter-proximal pausing
dc.creator | Jimeno González, Silvia | es |
dc.creator | Ceballos Chávez, María | es |
dc.creator | Reyes , José Carlos | es |
dc.date.accessioned | 2017-03-14T13:03:21Z | |
dc.date.available | 2017-03-14T13:03:21Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Jimeno González, S., Ceballos Chávez, M. y Reyes , J.C. (2015). A positioned +1 nucleosome enhances promoter-proximal pausing. Nucleic Acids Research, 43 (6), 3068-3078. | |
dc.identifier.issn | 0305-1048 | es |
dc.identifier.uri | http://hdl.handle.net/11441/55834 | |
dc.description.abstract | Chromatin distribution is not uniform along the human genome. In most genes there is a promoter-associated nucleosome free region (NFR) followed by an array of nucleosomes towards the gene body in which the first (+1) nucleosome is strongly positioned. The function of this characteristic chromatin distribution in transcription is not fully understood. Here we show in vivo that the +1 nucleosome plays a role in modulating RNA polymerase II (RNAPII) promoter-proximal pausing. When a +1 nucleosome is strongly positioned, elongating RNAPII has a tendency to stall at the promoter-proximal region, recruits more negative elongation factor (NELF) and produces less mRNA. The nucleosome-induced pause favors pre-mRNA quality control by promoting the addition of the cap to the nascent RNA. Moreover, the uncapped RNAs produced in the absence of a positioned nucleosome are degraded by the 5′-3′ exonuclease XRN2. Interestingly, reducing the levels of the chromatin remodeler ISWI factor SNF2H decreases +1 nucleosome positioning and increases RNAPII pause release. This work demonstrates a function for +1 nucleosome in regulation of transcription elongation, pre-mRNA processing and gene expression. | es |
dc.description.sponsorship | España, Ministerio de Economía y Competitividad BFU- 2011-23442 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Oxford University Press | es |
dc.relation.ispartof | Nucleic Acids Research, 43 (6), 3068-3078. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | A positioned +1 nucleosome enhances promoter-proximal pausing | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) | es |
dc.relation.projectID | BFU-2011-23442 | es |
dc.relation.publisherversion | 10.1093/nar/gkv149 | es |
dc.identifier.doi | 10.1093/nar/gkv149 | es |
idus.format.extent | 12 p. | es |
dc.journaltitle | Nucleic Acids Research | es |
dc.publication.volumen | 43 | es |
dc.publication.issue | 6 | es |
dc.publication.initialPage | 3068 | es |
dc.publication.endPage | 3078 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España |
Ficheros | Tamaño | Formato | Ver | Descripción |
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pub34gkv149.pdf | 3.462Mb | [PDF] | Ver/ | |