Show simple item record

Article

dc.creatorJimeno González, Silviaes
dc.creatorCeballos Chávez, Maríaes
dc.creatorReyes , José Carloses
dc.date.accessioned2017-03-14T13:03:21Z
dc.date.available2017-03-14T13:03:21Z
dc.date.issued2015
dc.identifier.citationJimeno González, S., Ceballos Chávez, M. y Reyes , J.C. (2015). A positioned +1 nucleosome enhances promoter-proximal pausing. Nucleic Acids Research, 43 (6), 3068-3078.
dc.identifier.issn0305-1048es
dc.identifier.urihttp://hdl.handle.net/11441/55834
dc.description.abstractChromatin distribution is not uniform along the human genome. In most genes there is a promoter-associated nucleosome free region (NFR) followed by an array of nucleosomes towards the gene body in which the first (+1) nucleosome is strongly positioned. The function of this characteristic chromatin distribution in transcription is not fully understood. Here we show in vivo that the +1 nucleosome plays a role in modulating RNA polymerase II (RNAPII) promoter-proximal pausing. When a +1 nucleosome is strongly positioned, elongating RNAPII has a tendency to stall at the promoter-proximal region, recruits more negative elongation factor (NELF) and produces less mRNA. The nucleosome-induced pause favors pre-mRNA quality control by promoting the addition of the cap to the nascent RNA. Moreover, the uncapped RNAs produced in the absence of a positioned nucleosome are degraded by the 5′-3′ exonuclease XRN2. Interestingly, reducing the levels of the chromatin remodeler ISWI factor SNF2H decreases +1 nucleosome positioning and increases RNAPII pause release. This work demonstrates a function for +1 nucleosome in regulation of transcription elongation, pre-mRNA processing and gene expression.es
dc.description.sponsorshipEspaña, Ministerio de Economía y Competitividad BFU- 2011-23442es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.ispartofNucleic Acids Research, 43 (6), 3068-3078.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleA positioned +1 nucleosome enhances promoter-proximal pausinges
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationCentro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER)es
dc.relation.projectIDBFU-2011-23442es
dc.relation.publisherversion10.1093/nar/gkv149es
dc.identifier.doi10.1093/nar/gkv149es
idus.format.extent12 p.es
dc.journaltitleNucleic Acids Researches
dc.publication.volumen43es
dc.publication.issue6es
dc.publication.initialPage3068es
dc.publication.endPage3078es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). España

FilesSizeFormatViewDescription
pub34gkv149.pdf3.462MbIcon   [PDF] View/Open  

This item appears in the following collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional