Article
SrfJ, a salmonella type III secretion system effector regulated by PhoP, RcsB, and IolR.
Author/s | Ramos Morales, Francisco
![]() ![]() ![]() ![]() ![]() ![]() ![]() Cordero Alba, Mar Bernal Bayard, Joaquín ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Department | Universidad de Sevilla. Departamento de Genética |
Publication Date | 2012 |
Deposit Date | 2016-02-26 |
Published in |
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Abstract | Virulence-related type III secretion systems are present in many Gram-negative bacterial pathogens. These complex devices
translocate proteins, called effectors, from the bacterium into the eukaryotic host cell. Here, we ... Virulence-related type III secretion systems are present in many Gram-negative bacterial pathogens. These complex devices translocate proteins, called effectors, from the bacterium into the eukaryotic host cell. Here, we identify the product of srfJ, a Salmonella enterica serovar Typhimurium gene regulated by SsrB, as a new substrate of the type III secretion system encoded by Salmonella pathogenicity island 2. The N-terminal 20-amino-acid segment of SrfJ was recognized as a functional secretion and translocation signal specific for this system. Transcription of srfJ was positively regulated by the PhoP/PhoQ system in an SsrBdependent manner and was negatively regulated by the Rcs system in an SsrB-independent manner. A screen for regulators of an srfJ-lacZ transcriptional fusion using the T-POP transposon identified IolR, the regulator of genes involved in myo-inositol utilization, as an srfJ repressor. Our results suggest that SrfJ is synthesized both inside the host, in response to intracellular conditions, and outside the host, in myo-inositol-rich environments. |
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