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Artículo
Carotid body chemosensory responses in mice deficient of TASK channels
dc.creator | Ortega Sáenz, Patricia | |
dc.creator | Levitski, Konstantín | |
dc.creator | Marcos Almaraz, María Teresa | |
dc.creator | Bonilla Henao, Victoria | |
dc.creator | Pascual Bravo, Alberto | |
dc.creator | López Barneo, José | |
dc.date.accessioned | 2015-01-15T10:46:45Z | |
dc.date.available | 2015-01-15T10:46:45Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 1540-7748 | es |
dc.identifier.issn | 0022-1295 | es |
dc.identifier.uri | http://hdl.handle.net/11441/17784 | |
dc.description.abstract | Background K+ channels of the TASK family are believed to participate in sensory transduction by chemoreceptor (glomus) cells of the carotid body (CB). However, studies on the systemic CB-mediated ventilatory response to hypoxia and hypercapnia in TASK1- and/or TASK3-deficient mice have yielded conflicting results. We have characterized the glomus cell phenotype of TASK-null mice and studied the responses of individual cells to hypoxia and other chemical stimuli. CB morphology and glomus cell size were normal in wild-type as well as in TASK1/ or double TASK1/3/ mice. Patch-clamped TASK1/3-null glomus cells had significantly higher membrane resistance and less hyperpolarized resting potential than their wild-type counterpart. These electrical parameters were practically normal in TASK1/ cells. Sensitivity of background currents to changes of extracellular pH was drastically diminished in TASK1/3-null cells. In contrast with these observations, responsiveness to hypoxia or hypercapnia of either TASK1/ or double TASK1/3/ cells, as estimated by the amperometric measurement of catecholamine release, was apparently normal. TASK1/3 knockout cells showed an enhanced secretory rate in basal (normoxic) conditions compatible with their increased excitability. Responsiveness to hypoxia of TASK1/3-null cells was maintained after pharmacological blockade of maxi-K+ channels. These data in the TASK-null mouse model indicate that TASK3 channels contribute to the background K+ current in glomus cells and to their sensitivity to external pH. They also suggest that, although TASK1 channels might be dispensable for O2/CO2 sensing in mouse CB cells, TASK3 channels (or TASK1/3 heteromers) could mediate hypoxic depolarization of normal glomus cells. The ability of TASK1/3/ glomus cells to maintain a powerful response to hypoxia even after blockade of maxi-K+ channels, suggests the existence of multiple sensor and/or effector mechanisms, which could confer upon the cells a high adaptability to maintain their chemosensory function. | es |
dc.language.iso | eng | es |
dc.relation.ispartof | The Journal of general physiology, 135 (4), 379-392. | es |
dc.rights | Atribución-NoComercial-SinDerivadas 4.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | Carotid body chemosensory responses in mice deficient of TASK channels | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.contributor.affiliation | Instituto de Biomedicina de Sevilla (IBIS) | |
dc.identifier.doi | 10.1085/jgp.200910302 | |
dc.journaltitle | The Journal of general physiology | es |
dc.publication.volumen | 135 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 379 | es |
dc.publication.endPage | 392 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/17784 |
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