Artículo
Symmetric and asymmetric DNA N6-adenine methylation regulates different biological responses in Mucorales
Autor/es | Lax, Carlos
Mondo, Stephen J. Osorio Concepción, Macario Muszewska, Anna Corrochano Luque, María ![]() ![]() ![]() ![]() ![]() Gutiérrez Pozo, Gabriel ![]() ![]() ![]() ![]() ![]() ![]() Cánovas López, David ![]() ![]() ![]() ![]() ![]() ![]() ![]() Corrochano Peláez, Luis María ![]() ![]() ![]() ![]() ![]() ![]() Garre, Victoriano |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2024-07-18 |
Fecha de depósito | 2024-07-23 |
Publicado en |
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Resumen | DNA N6-adenine methylation (6mA) has recently gained importance as an epigenetic modification in eukaryotes. Its function in lineages with high levels, such as early-diverging fungi (EDF), is of particular interest. Here, ... DNA N6-adenine methylation (6mA) has recently gained importance as an epigenetic modification in eukaryotes. Its function in lineages with high levels, such as early-diverging fungi (EDF), is of particular interest. Here, we investigated the biological significance and evolutionary implications of 6mA in EDF, which exhibit divergent evolutionary patterns in 6mA usage. The analysis of two Mucorales species displaying extreme 6mA usage reveals that species with high 6mA levels show symmetric methylation enriched in highly expressed genes. In contrast, species with low 6mA levels show mostly asymmetric 6mA. Interestingly, transcriptomic regulation throughout development and in response to environmental cues is associated with changes in the 6mA landscape. Furthermore, we identify an EDF-specific methyltransferase, likely originated from endosymbiotic bacteria, as responsible for asymmetric methylation, while an MTA-70 methylation complex performs symmetric methylation. The distinct phenotypes observed in the corresponding mutants reinforced the critical role of both types of 6mA in EDF. |
Agencias financiadoras | Ministerio de Ciencia e Innovación (MICIN). España Agencia Estatal de Investigación. España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Comunidad Autónoma de Murcia. España Department of Energy Joint Genome Institute. U. S. Office of Science of the US Department of Energy. U. S. |
Identificador del proyecto | PID2021-124674NB-I00
![]() PID2021-128001OB-I00 ![]() 20897/PI/18 ![]() 21969/PI/22 ![]() P20-00622 ![]() |
Cita | Lax, C., Mondo, S.J., Osorio Concepción, M., Muszewska, A., Corrochano Luque, M., Gutiérrez Pozo, G.,...,Garre, V. (2024). Symmetric and asymmetric DNA N6-adenine methylation regulates different biological responses in Mucorales. Nature Communications, 15 (1), 6066. https://doi.org/10.1038/s41467-024-50365-2. |
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