dc.creator | Casas Rodríguez, Antonio | es |
dc.creator | Medrano Padial, Concepción | es |
dc.creator | Jos Gallego, Ángeles Mencía | es |
dc.creator | Cameán Fernández, Ana María | es |
dc.creator | Campos, Alexandre | es |
dc.creator | Fonseca, Elza | es |
dc.date.accessioned | 2024-07-12T13:32:05Z | |
dc.date.available | 2024-07-12T13:32:05Z | |
dc.date.issued | 2024-06-07 | |
dc.identifier.citation | Casas Rodríguez, A., Medrano Padial, C., Jos Gallego, Á.M., Cameán Fernández, A.M., Campos, A. y Fonseca, E. (2024). Characterization of NR1J1 Paralog Responses of Marine Mussels: Insights from Toxins and Natural Activators. International Journal of Molecular Sciences, 25 (12), 6287. https://doi.org/10.3390/ijms25126287. | |
dc.identifier.issn | 1422-0067 | es |
dc.identifier.uri | https://hdl.handle.net/11441/161344 | |
dc.description.abstract | The pregnane X receptor (PXR) is a nuclear hormone receptor that plays a pivotal role in regulating gene expression in response to various ligands, particularly xenobiotics. In this context, the aim of this study was to shed light on the ligand affinity and functions of four NR1J1 paralogs identified in the marine mussel Mytilus galloprovincialis, employing a dual-luciferase reporter assay. To achieve this, the activation patterns of these paralogs in response to various toxins, including freshwater cyanotoxins (Anatoxin-a, Cylindrospermopsin, and Microcystin-LR, -RR, and -YR) and marine algal toxins (Nodularin, Saxitoxin, and Tetrodotoxin), alongside natural compounds (Saint John’s Wort, Ursolic Acid, and 8-Methoxypsoralene) and microalgal extracts (Tetraselmis, Isochrysis, LEGE 95046, and LEGE 91351 extracts), were studied. The investigation revealed nuanced differences in paralog response patterns, highlighting the remarkable sensitivity of MgaNR1J1γ and MgaNR1J1δ paralogs to several toxins. In conclusion, this study sheds light on the intricate mechanisms of xenobiotic metabolism and detoxification, particularly focusing on the role of marine mussel NR1J1 in responding to a diverse array of compounds. Furthermore, comparative analysis with human PXR revealed potential species-specific adaptations in detoxification mechanisms, suggesting evolutionary implications. These findings deepen our understanding of PXR-mediated metabolism mechanisms, offering insights into environmental monitoring and evolutionary biology research. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID 2019-104890RB-I00 | es |
dc.description.sponsorship | European Union 823860, 101086234 | es |
dc.description.sponsorship | Portuguese Foundation for Science and Technology UIDB/04423/2020, UIDP/04423/2020, CEECIND/03767/2018 | es |
dc.format | application/pdf | es |
dc.format.extent | 16 p. | es |
dc.language.iso | eng | es |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es |
dc.relation.ispartof | International Journal of Molecular Sciences, 25 (12), 6287. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Bivalves | es |
dc.subject | Cyanotoxins | es |
dc.subject | Marine mussels | es |
dc.subject | Microalgal extracts | es |
dc.subject | NR1J1 paralogs | es |
dc.subject | Pregnane X receptor | es |
dc.subject | Toxins | es |
dc.title | Characterization of NR1J1 Paralog Responses of Marine Mussels: Insights from Toxins and Natural Activators | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal | es |
dc.relation.projectID | PID 2019-104890RB-I00 | es |
dc.relation.projectID | 823860 | es |
dc.relation.projectID | 101086234 | es |
dc.relation.projectID | UIDB/04423/2020 | es |
dc.relation.projectID | UIDP/04423/2020 | es |
dc.relation.projectID | CEECIND/03767/2018 | es |
dc.relation.publisherversion | https://doi.org/10.3390/ijms25126287 | es |
dc.identifier.doi | 10.3390/ijms25126287 | es |
dc.journaltitle | International Journal of Molecular Sciences | es |
dc.publication.volumen | 25 | es |
dc.publication.issue | 12 | es |
dc.publication.initialPage | 6287 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | European Union (UE) | es |
dc.contributor.funder | Foundation for Science and Technology. Portugal | es |