Article
Characterization of NR1J1 Paralog Responses of Marine Mussels: Insights from Toxins and Natural Activators
Author/s | Casas Rodríguez, Antonio
Medrano Padial, Concepción Jos Gallego, Ángeles Mencía Cameán Fernández, Ana María Campos, Alexandre Fonseca, Elza |
Department | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal |
Publication Date | 2024-06-07 |
Deposit Date | 2024-07-12 |
Published in |
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Abstract | The pregnane X receptor (PXR) is a nuclear hormone receptor that plays a pivotal role in regulating gene expression in response to various ligands, particularly xenobiotics. In this context, the aim of this study was to ... The pregnane X receptor (PXR) is a nuclear hormone receptor that plays a pivotal role in regulating gene expression in response to various ligands, particularly xenobiotics. In this context, the aim of this study was to shed light on the ligand affinity and functions of four NR1J1 paralogs identified in the marine mussel Mytilus galloprovincialis, employing a dual-luciferase reporter assay. To achieve this, the activation patterns of these paralogs in response to various toxins, including freshwater cyanotoxins (Anatoxin-a, Cylindrospermopsin, and Microcystin-LR, -RR, and -YR) and marine algal toxins (Nodularin, Saxitoxin, and Tetrodotoxin), alongside natural compounds (Saint John’s Wort, Ursolic Acid, and 8-Methoxypsoralene) and microalgal extracts (Tetraselmis, Isochrysis, LEGE 95046, and LEGE 91351 extracts), were studied. The investigation revealed nuanced differences in paralog response patterns, highlighting the remarkable sensitivity of MgaNR1J1γ and MgaNR1J1δ paralogs to several toxins. In conclusion, this study sheds light on the intricate mechanisms of xenobiotic metabolism and detoxification, particularly focusing on the role of marine mussel NR1J1 in responding to a diverse array of compounds. Furthermore, comparative analysis with human PXR revealed potential species-specific adaptations in detoxification mechanisms, suggesting evolutionary implications. These findings deepen our understanding of PXR-mediated metabolism mechanisms, offering insights into environmental monitoring and evolutionary biology research. |
Funding agencies | Ministerio de Ciencia e Innovación (MICIN). España European Union (UE) Foundation for Science and Technology. Portugal |
Project ID. | PID 2019-104890RB-I00
823860 101086234 UIDB/04423/2020 UIDP/04423/2020 CEECIND/03767/2018 |
Citation | Casas Rodríguez, A., Medrano Padial, C., Jos Gallego, Á.M., Cameán Fernández, A.M., Campos, A. y Fonseca, E. (2024). Characterization of NR1J1 Paralog Responses of Marine Mussels: Insights from Toxins and Natural Activators. International Journal of Molecular Sciences, 25 (12), 6287. https://doi.org/10.3390/ijms25126287. |
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