Article
The RNAi Machinery in the Fungus Fusarium fujikuroi Is Not Very Active in Synthetic Medium and Is Related to Transposable Elements
Author/s | Pardo Medina, Javier
![]() ![]() ![]() ![]() ![]() ![]() ![]() Dahlmann, Tim A. Nowrousian, Minou Limón Mirón, María del Carmen ![]() ![]() ![]() ![]() ![]() ![]() ![]() Ávalos Cordero, Francisco Javier ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Department | Universidad de Sevilla. Departamento de Genética |
Publication Date | 2024-05-16 |
Deposit Date | 2024-07-11 |
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Abstract | Small RNAS (sRNAs) participate in regulatory RNA interference (RNAi) mechanisms in a wide range of eukaryotic organisms, including fungi. The fungus Fusarium fujikuroi, a model for the study of secondary metabolism, contains ... Small RNAS (sRNAs) participate in regulatory RNA interference (RNAi) mechanisms in a wide range of eukaryotic organisms, including fungi. The fungus Fusarium fujikuroi, a model for the study of secondary metabolism, contains a complete set of genes for RNAi pathways. We have analyzed by high-throughput sequencing the content of sRNAs in total RNA samples of F. fujikuroi grown in synthetic medium in the dark or after 1 h of illumination, using libraries below 150 nt, covering sRNAs and their precursors. For comparison, a parallel analysis with Fusarium oxysporum was carried out. The sRNA reads showed a higher proportion of 5′ uracil in the RNA samples of the expected sizes in both species, indicating the occurrence of genuine sRNAs, and putative miRNA-like sRNAs (milRNAS) were identified with prediction software. F. fujikuroi carries at least one transcriptionally expressed Ty1/copia-like retrotransposable element, in which sRNAs were found in both sense and antisense DNA strands, while in F. oxysporum skippy-like elements also show sRNA formation. The finding of sRNA in these mobile elements indicates an active sRNA-based RNAi pathway. Targeted deletion of dcl2, the only F. fujikuroi Dicer gene with significant expression under the conditions tested, did not produce appreciable phenotypic or transcriptomic alterations. |
Funding agencies | Ministerio de Ciencia e Innovación (MICIN). España |
Project ID. | PID2022-140414NB-I00
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Citation | Pardo Medina, J., Dahlmann, T.A., Nowrousian, M., Limón Mirón, M.d.C. y Ávalos Cordero, F.J. (2024). The RNAi Machinery in the Fungus Fusarium fujikuroi Is Not Very Active in Synthetic Medium and Is Related to Transposable Elements. Non-coding RNA, 10 (3), 31. https://doi.org/10.3390/ncrna10030031. |
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