dc.creator | Pardo Medina, Javier | es |
dc.creator | Dahlmann, Tim A. | es |
dc.creator | Nowrousian, Minou | es |
dc.creator | Limón Mirón, María del Carmen | es |
dc.creator | Ávalos Cordero, Francisco Javier | es |
dc.date.accessioned | 2024-07-11T13:30:50Z | |
dc.date.available | 2024-07-11T13:30:50Z | |
dc.date.issued | 2024-05-16 | |
dc.identifier.citation | Pardo Medina, J., Dahlmann, T.A., Nowrousian, M., Limón Mirón, M.d.C. y Ávalos Cordero, F.J. (2024). The RNAi Machinery in the Fungus Fusarium fujikuroi Is Not Very Active in Synthetic Medium and Is Related to Transposable Elements. Non-coding RNA, 10 (3), 31. https://doi.org/10.3390/ncrna10030031. | |
dc.identifier.issn | 2311-553X | es |
dc.identifier.uri | https://hdl.handle.net/11441/161310 | |
dc.description.abstract | Small RNAS (sRNAs) participate in regulatory RNA interference (RNAi) mechanisms in a wide range of eukaryotic organisms, including fungi. The fungus Fusarium fujikuroi, a model for the study of secondary metabolism, contains a complete set of genes for RNAi pathways. We have analyzed by high-throughput sequencing the content of sRNAs in total RNA samples of F. fujikuroi grown in synthetic medium in the dark or after 1 h of illumination, using libraries below 150 nt, covering sRNAs and their precursors. For comparison, a parallel analysis with Fusarium oxysporum was carried out. The sRNA reads showed a higher proportion of 5′ uracil in the RNA samples of the expected sizes in both species, indicating the occurrence of genuine sRNAs, and putative miRNA-like sRNAs (milRNAS) were identified with prediction software. F. fujikuroi carries at least one transcriptionally expressed Ty1/copia-like retrotransposable element, in which sRNAs were found in both sense and antisense DNA strands, while in F. oxysporum skippy-like elements also show sRNA formation. The finding of sRNA in these mobile elements indicates an active sRNA-based RNAi pathway. Targeted deletion of dcl2, the only F. fujikuroi Dicer gene with significant expression under the conditions tested, did not produce appreciable phenotypic or transcriptomic alterations. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2022-140414NB-I00 | es |
dc.format | application/pdf | es |
dc.format.extent | 18 p. | es |
dc.language.iso | eng | es |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es |
dc.relation.ispartof | Non-coding RNA, 10 (3), 31. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Dicer gene | es |
dc.subject | Fusarium fujikuroi | es |
dc.subject | Fusarium oxysporum | es |
dc.subject | High-throughput sequencing | es |
dc.subject | RNA interference | es |
dc.subject | sRNA | es |
dc.subject | Transposable elements | es |
dc.title | The RNAi Machinery in the Fungus Fusarium fujikuroi Is Not Very Active in Synthetic Medium and Is Related to Transposable Elements | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | PID2022-140414NB-I00 | es |
dc.relation.publisherversion | https://doi.org/10.3390/ncrna10030031 | es |
dc.identifier.doi | 10.3390/ncrna10030031 | es |
dc.journaltitle | Non-coding RNA | es |
dc.publication.volumen | 10 | es |
dc.publication.issue | 3 | es |
dc.publication.initialPage | 31 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |