dc.creator | Gomez Muñoz, Maria De Los Angeles | es |
dc.creator | Aguilar Morante, Diana | es |
dc.creator | Colmenero-Repiso, Ana | es |
dc.creator | Amador Álvarez, Aida | es |
dc.creator | Ojeda-Puertas, Mónica | es |
dc.creator | Cordero Varela, Juan Antonio | es |
dc.creator | Rodríguez-Prieto, Ismael | es |
dc.creator | Pardal Redondo, Ricardo | es |
dc.creator | Vega Moreno, Francisco Manuel | es |
dc.date.accessioned | 2023-06-07T13:20:17Z | |
dc.date.available | 2023-06-07T13:20:17Z | |
dc.date.issued | 2023-01-13 | |
dc.identifier.citation | Gomez Muñoz, M.D.L.A., Aguilar Morante, D., Colmenero-Repiso, A., Amador Álvarez, A., Ojeda-Puertas, M., Cordero Varela, J.A.,...,Vega Moreno, F.M. (2023). Analysis of serial neuroblastoma PDX passages in mice allows the identification of new mediators of neuroblastoma aggressiveness. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (2), 1590. https://doi.org/10.3390/ijms24021590. | |
dc.identifier.issn | 1422-0067 | es |
dc.identifier.uri | https://hdl.handle.net/11441/147009 | |
dc.description.abstract | Neuroblastoma is a neural crest cell-derived pediatric tumor characterized by high interand intra-tumor heterogeneity, and by a poor outcome in advanced stages. Patient-derived xenografts
(PDXs) have been shown to be useful models for preserving and expanding original patient biopsies
in vivo, and for studying neuroblastoma biology in a more physiological setting. The maintenance of
genetic, histologic, and phenotypic characteristics of the original biopsy along serial PDX passages
in mice is a major concern regarding this model. Here we analyze consecutive PDX passages
in mice, at both transcriptomic and histological levels, in order to identify potential changes or
highlight similarities to the primary sample. We studied temporal changes using mRNA and miRNA
expression and correlate those with neuroblastoma aggressiveness using patient-derived databases.
We observed a shortening of tumor onset and an increase in proliferative potential in the PDXs
along serial passages. This behavior correlates with changes in the expression of genes related to
cell proliferation and neuronal differentiation, including signaling pathways described as relevant
for neuroblastoma malignancy. We also identified new genes and miRNAs that can be used to
stratify patients according to survival, and which could be potential new players in neuroblastoma
aggressiveness. Our results highlight the usefulness of the PDX neuroblastoma model and reflect
phenotypic changes that might be occurring in the mouse environment. These findings could be
useful for understanding the progression of tumor aggressiveness in this pathology | es |
dc.format | application/pdf | es |
dc.format.extent | 15 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (2), 1590. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Neuroblastoma | es |
dc.subject | PDX | es |
dc.subject | Gene expression | es |
dc.subject | miRNA | es |
dc.subject | Differentiation | es |
dc.title | Analysis of serial neuroblastoma PDX passages in mice allows the identification of new mediators of neuroblastoma aggressiveness | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.relation.projectID | P18-RT-3151 | es |
dc.relation.projectID | US-1262985 | es |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/24/2/1590 | es |
dc.identifier.doi | 10.3390/ijms24021590 | es |
dc.journaltitle | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | es |
dc.publication.volumen | 24 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 1590 | es |
dc.contributor.funder | Junta de Andalucía-Universidad de Sevilla | es |