dc.creator | Ruiz Laza, Rocío | es |
dc.creator | Cano, Raquel | es |
dc.creator | Casañas, Juan José | es |
dc.creator | Gaffield, Michael A. | es |
dc.creator | Betz, William J. | es |
dc.creator | Tabares, Lucía | es |
dc.date.accessioned | 2023-05-19T13:16:49Z | |
dc.date.available | 2023-05-19T13:16:49Z | |
dc.date.issued | 2011-02-09 | |
dc.identifier.citation | Ruiz Laza, R., Cano, R., Casañas, J.J., Gaffield, M.A., Betz, W.J. y Tabares, L. (2011). Active zones and the readily releasable pool of synaptic vesicles at the neuromuscular junction of the mouse. JOURNAL OF NEUROSCIENCE, 31 (6), 200-2008. https://doi.org/10.1523/JNEUROSCI.4663-10.2011. | |
dc.identifier.issn | 0270-6474 | es |
dc.identifier.uri | https://hdl.handle.net/11441/146455 | |
dc.description.abstract | Synchronous neurotransmitter releaseis a highly regulated processthattakes place at specializations atthe presynapticmembrane called
active zones (AZs). The relationships between AZs, quantal release, and vesicle replenishment are not well understood in a mature
synapse. We have measured the number, distribution, and other properties of AZs in mouse motor nerve terminals and combined these
observations with electrophysiological estimates of the size of the readily releasable pool (RRP) of synaptic vesicles. On average, we
counted 850 AZs per terminal. Assuming two primary docked vesicles per AZ, we predict a total of 1700 vesicles optimally positioned
for exocytosis. Electrophysiological estimates of the size of the RRP, using a simple kinetic model that assumes exponential depletion of
the initial pool and refilling by recruitment, gave an average value of 1730 quanta during 100 Hz stimulation, in satisfying agreement with
the morphology. At lower stimulus frequencies, however, the model revealed that the estimated RRP size is smaller, suggesting that not
all AZs participate in release at low stimulation frequencies. | es |
dc.format | application/pdf | es |
dc.format.extent | 9 p. | es |
dc.language.iso | eng | es |
dc.publisher | SOC NEUROSCIENCE | es |
dc.relation.ispartof | JOURNAL OF NEUROSCIENCE, 31 (6), 200-2008. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Active zones and the readily releasable pool of synaptic vesicles at the neuromuscular junction of the mouse | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.relation.publisherversion | https://www.jneurosci.org/content/31/6/2000 | es |
dc.identifier.doi | 10.1523/JNEUROSCI.4663-10.2011 | es |
dc.journaltitle | JOURNAL OF NEUROSCIENCE | es |
dc.publication.volumen | 31 | es |
dc.publication.issue | 6 | es |
dc.publication.initialPage | 200 | es |
dc.publication.endPage | 2008 | es |