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dc.creatorRuiz Laza, Rocíoes
dc.creatorCano, Raqueles
dc.creatorCasañas, Juan Josées
dc.creatorGaffield, Michael A.es
dc.creatorBetz, William J.es
dc.creatorTabares, Lucíaes
dc.date.accessioned2023-05-19T13:16:49Z
dc.date.available2023-05-19T13:16:49Z
dc.date.issued2011-02-09
dc.identifier.citationRuiz Laza, R., Cano, R., Casañas, J.J., Gaffield, M.A., Betz, W.J. y Tabares, L. (2011). Active zones and the readily releasable pool of synaptic vesicles at the neuromuscular junction of the mouse. JOURNAL OF NEUROSCIENCE, 31 (6), 200-2008. https://doi.org/10.1523/JNEUROSCI.4663-10.2011.
dc.identifier.issn0270-6474es
dc.identifier.urihttps://hdl.handle.net/11441/146455
dc.description.abstractSynchronous neurotransmitter releaseis a highly regulated processthattakes place at specializations atthe presynapticmembrane called active zones (AZs). The relationships between AZs, quantal release, and vesicle replenishment are not well understood in a mature synapse. We have measured the number, distribution, and other properties of AZs in mouse motor nerve terminals and combined these observations with electrophysiological estimates of the size of the readily releasable pool (RRP) of synaptic vesicles. On average, we counted 850 AZs per terminal. Assuming two primary docked vesicles per AZ, we predict a total of 1700 vesicles optimally positioned for exocytosis. Electrophysiological estimates of the size of the RRP, using a simple kinetic model that assumes exponential depletion of the initial pool and refilling by recruitment, gave an average value of 1730 quanta during 100 Hz stimulation, in satisfying agreement with the morphology. At lower stimulus frequencies, however, the model revealed that the estimated RRP size is smaller, suggesting that not all AZs participate in release at low stimulation frequencies.es
dc.formatapplication/pdfes
dc.format.extent9 p.es
dc.language.isoenges
dc.publisherSOC NEUROSCIENCEes
dc.relation.ispartofJOURNAL OF NEUROSCIENCE, 31 (6), 200-2008.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleActive zones and the readily releasable pool of synaptic vesicles at the neuromuscular junction of the mousees
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica y Biología Moleculares
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Fisiología Médica y Biofísicaes
dc.relation.publisherversionhttps://www.jneurosci.org/content/31/6/2000es
dc.identifier.doi10.1523/JNEUROSCI.4663-10.2011es
dc.journaltitleJOURNAL OF NEUROSCIENCEes
dc.publication.volumen31es
dc.publication.issue6es
dc.publication.initialPage200es
dc.publication.endPage2008es

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